Determination of binding constant and binding region of programmed cell death 5-heparin by capillary zone electrophoresis.

Full Abstract

A sensitive and selective high-performance analytical method based on capillary zone electrophoresis (CZE) was developed for investigating interactions between heparin and programmed cell death 5 (PDCD5) qualitatively and quantitatively. The binding constant of the interaction between PDCD5 and heparin calculated by Scatchard analysis was 4.17x10(4) M(-1) and the binding sites located in the C-terminal region of PDCD5 (residues 109-115). The precisions of migration times, peak heights and binding constants, expressed as the relative standard deviation, were less than 2.4%, 1.1% and 5.7%, respectively.

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Author information

Author/s: Yi, Liu (L); Xiaomei, Ling (L); Hui, Fan (F); Yingcheng, Zhong (Z); Dan, Li (L); Ying, Wang (W);

Affiliation: Department of Pharmaceutical Analysis, School of Pharmaceutical Sciences, Peking University, Beijing 100083, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2007-Mar; vol 1143 (issue 1-2) : pp 284-7

Dates: Created 2007/02/12; Completed 2007/07/03;

PMID: 17257606, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence
Apoptosis Regulatory Proteins - chemistry; metabolism
Electrophoresis, Capillary - methods
Heparin - metabolism

Molecular Sequence Data
Neoplasm Proteins - chemistry; metabolism
Protein Binding
Sensitivity and Specificity

Associated Chemicals: Apoptosis Regulatory Proteins (0) ; Neoplasm Proteins (0) ; PDCD5 protein, human (0) ; Heparin (9005-49-6)

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