Scanning holographic microscopy with resolution exceeding the Rayleigh limit of the objective by superposition of off-axis holograms.

Full Abstract

We present what we believe to be a new application of scanning holographic microscopy to superresolution. Spatial resolution exceeding the Rayleigh limit of the objective is obtained by digital coherent addition of the reconstructions of several off-axis Fresnel holograms. Superresolution by holographic superposition and synthetic aperture has a long history, which is briefly reviewed. The method is demonstrated experimentally by combining three off-axis holograms of fluorescent beads showing a transverse resolution gain of nearly a factor of 2.

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Author information

Author/s: Indebetouw, Guy (G); Tada, Yoshitaka (Y); Rosen, Joseph (J); Brooker, Gary (G);

Affiliation: Department of Physics, Virginia Tech, Blacksburg, Virginia 24061-0435, USA. gindebet(-atsign-)vt.edu

Grants: R21 RR018440 (Agency:NCRR NIH HHS)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Applied optics (Appl Opt), published in United States. (Language: eng)

Reference: 2007-Feb; vol 46 (issue 6) : pp 993-1000

Dates: Created 2007/02/06; Completed 2007/03/30; Revised 2007/12/03;

PMID: 17279147, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Holography - instrumentation; methods
Image Processing, Computer-Assisted

Microscopy - instrumentation; methods
Models, Theoretical

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