[Study on extraction process of polysaccharide from Sargassum fusiforme by enzymatic treatment]

Full Abstract

OBJECTIVE:
To investigate the optimal extraction process of polysaccharides from S. fusiforme by enzymatic treatment.

METHOD:
The optimum extraction conditions were obtained by the experiment with the orthogonal design. The content of polysaccharides of S. fusiforme was determined by spectraphotometry. RESULT:The amount of enzyme and temperature significantly affected total polysaccharides of S. fusiforme.

CONCLUSION:
The optimum extraction conditions include the addition of 1. 2 x 10 (4) U x 100 g(-1) enzyme into water at pH 4. 5, and the subsequent treatment for 10 min while the temperature is maintained at 45 degrees C.

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Author information

Author/s: Zhang, Hua-fang (HF); Jin, Jing-shun (JS); Tan, Rong-mei (RM); Shen, Cheng (C);

Affiliation: School of Medicine, Shaoxing University, Shaoxing 312000, China. huafang_zhang(-atsign-)hotmail.com

Journal and publication information

Publication Type: English Abstract; Journal Article; Research Support, Non-U.S. Gov't

Journal: Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica (Zhongguo Zhong Yao Za Zhi), published in China. (Language: chi)

Reference: 2006-Nov; vol 31 (issue 22) : pp 1860-2

Dates: Created 2007/02/08; Completed 2008/04/24;

PMID: 17285983, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Cellulase - metabolism
Hydrogen-Ion Concentration
Polysaccharides - analysis; isolation & purification; metabolism

Sargassum - chemistry
Technology, Pharmaceutical - methods
Temperature

Associated Chemicals: Polysaccharides (0) ; Cellulase (EC 3.2.1.4)

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