[Primary application of spectral karyotyping in leukemia]

Full Abstract

OBJECTIVE:
To establish a spectral karyotyping (SKY) technique and explore the value of SKY in leukemia research.

METHODS:
SKY technique was conducted on 2 samples of peripheral blood from 2 healthy volunteers, then on the samples from 8 patients with leukemia, including chronic myelocytic leukemia (CML) and acute myelocytic leukemia (AML) confirmed by R-banding. In addition, four patients underwent dual fusion-fluorescence in situ hybridization (DF-FISH) to detect the mixed lineage leukemia (MLL), PML/RARa, and BCR/ABL fusion genes. By comparing the results of SKY, R-band karyotyping, and DF-FISH, the stability and reliability of SKY was judged.

RESULTS:
All 10 samples were successfully hybridized and karyotyped. The 2 cases of healthy volunteers showed normal karyotypes, thus, a specific SKY technique was successfully established. In the 8 cases of leukemia patients, SKY identified aberrations including 9q-, t (9; 22), t (15; 17) and the complex karyotype 47, XY, +9?ins (1;5) (q23;q23), t (6;7) (q23?; p13), in addition, the SKY technique detected some number abnormalities. The results of SKY confirmed the results of R-band karyotyping and DF-FISH; moreover, the SKY technique provided more accurate karyotypes.

CONCLUSION:
With high stability, accuracy, and sensitivity, the SKY technique established by this study can be applied in leukemia research.

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Author information

Author/s: Guo, Bo (B); DA, Wan-ming (WM); Han, Xiao-ping (XP); Sun, Jing-fen (JF); Huang, Wen-rong (WR); Wang, Ke (K);

Affiliation: General Hospital of People's Liberation Army, Beijing 100853, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhonghua yi xue za zhi (Zhonghua Yi Xue Za Zhi), published in China. (Language: chi)

Reference: 2006-Nov; vol 86 (issue 42) : pp 2978-81

Dates: Created 2007/02/09; Completed 2008/05/05;

PMID: 17288810, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult
Aged
Chromosome Banding - methods
Female
Humans
In Situ Hybridization, Fluorescence
Leukemia - diagnosis; genetics

Male
Middle Aged
Oncogene Proteins, Fusion - genetics
Reproducibility of Results
Sensitivity and Specificity
Spectral Karyotyping - methods
Translocation, Genetic

Associated Chemicals: Oncogene Proteins, Fusion (0)

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