Design and performance of an atmospheric pressure ion mobility Fourier transform ion cyclotron resonance mass spectrometer.

Full Abstract

This manuscript presents our initial results and development of a novel hybrid instrument that combines atmospheric pressure ion mobility spectrometry (AP-IMS) with Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS). Our preliminary results obtained from atmospheric pressure mobility separation of peptide mixtures combined with high-resolution FTICR mass analysis are demonstrated. The custom IMS system was constructed in-house and was coupled to the commercial FTICR-MS instrument through a flared inlet capillary interface. Dual-gate ion filtration was adapted to allow concurrent measurement of both mobility and m/z values. The feasibility of mobility separation was demonstrated with baseline separation of the peptides bradykinin and angiotensin II and their measured reduced mobility constants which were consistent with those previously reported. Furthermore, the unique size-to-charge separation mechanism of IMS that allows isomer separation was explored and demonstrated with the partial separation of two isomeric phosphopeptides. We feel the combination of IMS and FTICR-MS holds great potential for accurate mass analysis of mobility-selected ions and these results are the first to demonstrate the feasibility of coupling these two techniques.Copyright (c) 2007 John Wiley & Sons, Ltd.

Learn Faster Today Improve your study skills

Author information

Author/s: Tang, Xiaoting (X); Bruce, James E (JE); Hill, Herbert H (HH);

Affiliation: Department of Chemistry, Washington State University, Pullman, WA 99164, USA.

Grants: R21 DK070274 (Agency:NIDDK NIH HHS)

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2007-; vol 21 (issue 7) : pp 1115-22

Dates: Created 2007/03/13; Completed 2007/05/29; Revised 2007/12/03;

PMID: 17318922, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Peptide Mapping - instrumentation; methods
Peptides - chemistry
Spectrometry, Mass, Electrospray Ionization - instrumentation; methods
Spectroscopy, Fourier Transform Infrared - instrumentation; methods

Associated Chemicals: Peptides (0)

These are the highest related articles currently in the database:

See 100+ related articles.