Multicenter evaluation of a rapid electrochemiluminescent adrenocorticotropic hormone (ACTH) immunoassay.

Full Abstract

BACKGROUND:
Measuring plasma adrenocorticotropic hormone (ACTH) is a key step in the differential diagnosis of hypothalamic-pituitary-adrenal disorders.

METHODS:
The recently developed electrochemiluminescence Elecsys ACTH immunoassay (Roche Diagnostics, Mannheim, Germany) was evaluated at six clinical laboratories on the Modular E170 and/or the Elecsys 2010 (Roche Diagnostics) immunoanalysers.

RESULTS:
The within-run and between-run imprecision was <or=1.9% and <or=5%, respectively (concentration level 12-971 ng/L). Functional sensitivity was at least 1.8 ng/L. The Elecsys assay correlated well with two established ACTH immunoradiometric assays (Brahms ACTH RIA and Nichols ACTH IRMA) and with the Immulite ACTH assay. Reference values for the Elecsys ACTH assay, determined as the percentile 5 to 95 (2.5 to 97.5) interval in 354 adults without disorder with impact on the hypothalamic-pituitary-adrenal axis, were between 7.2 and 63 ng/L (5.6 and 76 ng/L). The clinical sensitivity of the Elecsys ACTH assay in 57 patients with confirmed disorders of the pituitary-adrenal axis was comparable to the established Brahms ACTH assay.

CONCLUSION:
The non-isotopic Elecsys ACTH immunoassay provides precision and reliability in combination with reduced turnaround time.

Learn Faster Today Improve your study skills

Author information

Author/s: Verschraegen, Inge (I); Anckaert, Ellen (E); Schiettecatte, Johan (J); Mees, Marleen (M); Garrido, Adolfo (A); Hermsen, Derik (D); Lentjes, Eef G W M (EG); Liebert, Andreas (A); Roth, Heinz-Jürgen (HJ); Stamminger, Gudrun (G); Smitz, Johan (J);

Affiliation: Laboratory of Radioimmunology, Dutch-speaking Free University of Brussels (AZ-VUB), Laarbeeklaan 101, B-1090 Brussels, Belgium.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Multicenter Study

Journal: Clinica chimica acta; international journal of clinical chemistry (Clin Chim Acta), published in Netherlands. (Language: eng)

Reference: 2007-May; vol 380 (issue 1-2) : pp 75-80

Dates: Created 2007/04/03; Completed 2007/07/19;

PMID: 17321508, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adrenal Gland Diseases - blood; diagnosis
Adrenocorticotropic Hormone - analysis
Adult
Aged
Aged, 80 and over
Chemiluminescent Measurements - instrumentation; methods; statistics & numerical data
Female
Humans

Humans
Immunoassay - instrumentation; methods; statistics & numerical data
Male
Middle Aged
Pituitary Diseases - blood; diagnosis
Pituitary-Adrenal System - metabolism; pathology
Reference Values
Sensitivity and Specificity

Associated Chemicals: Adrenocorticotropic Hormone (9002-60-2)

These are the highest related articles currently in the database:

See 100+ related articles.