Research article summary (published 9 Feb 2007):

Differentiation of five tuna species by a multiplex primer-extension assay.

Full Abstract

A novel methodology based on analysis of mtDNA-cytb diagnostic sites was performed to discriminate four closely related species of Thunnus (Thunnus alalunga, Thunnus albacares, Thunnus obesus and Thunnus thynnus) and one species of Euthynnus (Katsuwonus pelamis) genus in raw and canned tuna. The primers used in the preliminary PCR designed in well conserved region upstream and downstream of the diagnosis sites successfully amplified a 132bp region from the cytb gene of all the species taken into consideration. The sites of diagnosis have been interrogate simultaneously using a multiplex primer-extension assay (PER) and the results were confirmed by fragment sequencing. The applicability of the multiplex PER assay to commercial canned tuna samples was also demonstrated. The proposed test could be useful for detection of fraud and for seafood traceability.

Author information

Author/s: Bottero, Maria Teresa (MT); Dalmasso, Alessandra (A); Cappelletti, Marco (M); Secchi, Camillo (C); Civera, Tiziana (T);

Affiliation: Department of Animal Pathology, Faculty of Veterinary Medicine, University of Torino, via Leonardo da Vinci 44, 10095 Grugliasco, Torino, Italy. mariateresa.bottero(-atsign-)unito.it

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of biotechnology (J Biotechnol), published in Netherlands. (Language: eng)

Reference: 2007-May; vol 129 (issue 3) : pp 575-80

Dates: Created 2007/04/16; Completed 2007/11/21; Revised 2008/11/21;

PMID: 17353060, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Base Sequence Cytochromes b - genetics DNA Primers DNA, Mitochondrial - genetics

Genetic Variation Molecular Sequence Data Sequence Analysis, DNA Species Specificity Tuna - genetics

Associated Chemicals: DNA Primers (0) ; DNA, Mitochondrial (0) ; Cytochromes b (9035-37-4)

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