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| Research article summary (published 8 Jan 2007): |
Determination of amino acids in saliva using capillary electrophoresis with fluorimetric detection.
Full Abstract
In the present study a sensitive method for the quantification of main free amino acids in saliva using capillary electrophoresis with laser induced fluorescence detection was developed. As background electrolyte 20 mM borate buffer pH 9.5 was used. Amino acids were separated after derivatization were optimized. The main amino acids occurring in saliva (Pro, Ser, Gly and Glu) were separated in less than 7 min. The parameters of validation such as linearity of response, precision and detection limits were determined. The detection limits were obtained in the range from 0.1 to 2.4 nM. The developed method was employed for determination of amino acids in real saliva samples.
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Author information
Author/s: Pobozy, Ewa (E); Czarkowska, Wioletta (W); Trojanowicz, Marek (M);
Affiliation: Departament of Chemistry, Warsaw University, Warsaw, Poland. ewapob(-atsign-)chem.uw.edu.pl
Journal and publication information
Publication Type: Journal Article
Journal: Journal of biochemical and biophysical methods (J Biochem Biophys Methods), published in Netherlands. (Language: eng)
Reference: 2007-Jan; vol 69 (issue 3) : pp XIII-XXIII
Dates: Created 2007/03/12; Completed 2007/03/29;
PMID: 17354274, status: MEDLINE (last retrieval date: 12/26/2008)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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