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| Research article summary (published 30 Jan 2007): |
A high throughput luminescent assay for glycogen synthase kinase-3beta inhibitors.
Full Abstract
A high throughput luminescent assay based on the Kinase-Glo() system (Promega, Madison, WI) has been developed for screening against glycogen synthase kinase-3beta (GSK-3beta). Careful optimization of assay parameters allowed us to develop a robust, reproducible, and sensitive assay. Its usefulness has been demonstrated in a high throughput screening run when screening 55,000 compounds. This campaign yielded five chemical classes of hits, including several highly potent GSK-3beta inhibitors.
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Author information
Author/s: Baki, Andrea (A); Bielik, Attila (A); Molnár, László (L); Szendrei, Györgyi (G); Keserü, György M (GM);
Affiliation: Chemical and Biotechnological R&D, Gedeon Richter Ltd., Budapest, Hungary.
Journal and publication information
Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't
Journal: Assay and drug development technologies (Assay Drug Dev Technol), published in United States. (Language: eng)
Reference: 2007-Feb; vol 5 (issue 1) : pp 75-83
Dates: Created 2007/03/14; Completed 2007/04/24;
PMID: 17355201, status: MEDLINE (last retrieval date: 12/26/2008)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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