Suppressive effect by Hizikia fusiforme on the production of tumor necrosis factor in BV2 murine microglial cells.

Full Abstract

BACKGROUND:
Hizikia fusiforme has been commonly used as food in Korea. Antioxidant effect of Hizikia fusiforme, however, was recently reported. Thus, herein, we investigated the effect of Hizikia fusiforme on the production and expression of tumor necrosis factor (TNF), a major proinflammatory mediator, in lipopolysaccharide (LPS)-activated BV2 microglial cells.

METHODS:
Cells were pre-treated with 5 or 50 mug/ml Hizikia fusiforme and treated with 1 mug/ml LPS. The production of TNF was measured by enzyme-linked immunosorbent assay (ELISA). The effect of Hizikia fusiforme on the expression of TNF was also performed by immunoblot analysis and reverse transcription-polymerase chain reaction (RT-PCR). Activation of nuclear factor kappab (NFkappab) was determined by electrophoretic mobility shift assay (EMSA).

RESULTS:
We observed that Hizikia fusiforme decreased the production of TNF. The inhibitory effect of the Hizikia fusiforme on the expression of TNF was confirmed by immunoblot and RT-PCR analyses. In addition, EMSA experiment revealed that Hizikia fusiforme blocked the LPS-induced activation of NFkappab.

CONCLUSION:
The present study suggests that Hizikia fusiforme may suppress LPS-stimulated TNF production via inhibition of NFkappab in murine microglial cells.

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Author information

Author/s: Jung, Kyunghee (K); Ha, Eunyoung (E); Uhm, Yoonkyung (Y); Park, Haejeong (H); Kim, Mi-Ja (MJ); Kim, Hyekyung (H); Baik, Hyunghwan (H); Hong, Meesuk (M); Yang, Jongsoo (J); Yim, Sung-Vin (SV);

Affiliation: Department of Pharmacology, College of Medicine, Kyung Hee University, Seoul 130-701, Korea.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Neurological research (Neurol Res), published in England. (Language: eng)

Reference: 2007-; vol 29 Suppl 1 (issue ) : pp S88-92

Dates: Created 2007/03/15; Completed 2007/05/15;

PMID: 17359647, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Cell Line
Dose-Response Relationship, Drug
Drug Interactions
Electrophoretic Mobility Shift Assay - methods
Enzyme-Linked Immunosorbent Assay
Gene Expression Regulation - drug effects
Lipopolysaccharides - pharmacology

Lipopolysaccharides - pharmacology
Mice
Microglia - drug effects; metabolism
Plant Extracts - pharmacology
Reverse Transcriptase Polymerase Chain Reaction - methods
Seaweed - chemistry
Tumor Necrosis Factor-alpha - metabolism
Tumor Necrosis Factors - genetics; metabolism

Associated Chemicals: Lipopolysaccharides (0) ; Plant Extracts (0) ; Tumor Necrosis Factor-alpha (0) ; Tumor Necrosis Factors (0)

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