A novel approach for a non competitive capillary electrophoresis immunoassay with laser-induced fluorescence detection for the determination of human serum albumin.

Full Abstract

A new non competitive capillary electrophoresis immunoassay format based on a separation into a capillary modified by analyte immobilisation is described. The injection of an excess of labelled antibody off-line preincubated with the analyte allows the surface capture of the free antibody and the immunocomplex detection. It was developed using the human serum albumin (HSA) as analyte, two FITC-labelled antibodies and a HSA covalently linked capillary. Two calibration curves with good run-to-run reproducibility and LOD--respectively 14.0 nM for the FITC-polyclonal antibody and 9.0 nM for the FITC-monoclonal antibody--were achieved. The assay was applied to HSA determination in spiked samples of human urine with acceptable recoveries.

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Author information

Author/s: Giovannoli, C (C); Anfossi, L (L); Baggiani, C (C); Giraudi, G (G);

Affiliation: Department of Analytical Chemistry, University of Torino, Torino, Italy. cristina.giovannoli@unito.it <cristina.giovannoli@unito.it>

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2007-Jul; vol 1155 (issue 2) : pp 187-92

Dates: Created 2007/06/18; Completed 2007/08/10;

PMID: 17359987, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Antibodies - immunology
Antigen-Antibody Complex
Electrophoresis, Capillary - methods
Fluorescent Dyes

Humans
Immunoassay - methods
Serum Albumin - analysis; immunology; isolation & purification
Spectrometry, Fluorescence

Associated Chemicals: Antibodies (0) ; Antigen-Antibody Complex (0) ; Fluorescent Dyes (0) ; Serum Albumin (0)

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