Immunization of nonautoimmune mice with DNA binding domains of the largest subunit of RNA polymerase I results in production of anti-dsDNA and anti-Sm/RNP antibodies.

Full Abstract

Antibodies against the N-terminal (NT) but not the basic domain (BD), DNA binding regions of the largest subunit (S1) of RNA polymerase I (RNAPI) were detected in the sera of MRL-lpr/lpr lupus mice. Antibodies against both RNAPI(S1)-NT and -BD, as well as other systemic lupus erythematosus (SLE) autoantigens (La, ribosomal P proteins and Sm/RNP) were produced by rabbits immunized with anti-DNA antibodies that had been affinity purified from SLE patients. Immunization of nonautoimmune mice (Balb/c) with RNAPI(S1)-NT, RNAPI(S1)-BD, or La in the form of GST fusion proteins, induced production of anti-double-stranded (ds) DNA and anti-Sm/RNP. GST-P1 did not induce an anti-dsDNA response in these mice. These results demonstrate that RNAPI(S1)-NT, RNAPI(S1)-BD and La can participate in an anti-autoantigen/anti-DNA antibody loop during an SLE-like autoimmune response.

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Author information

Author/s: Sciascia, Sandra A (SA); Robson, Kristina (K); Zhu, Liangjin (L); Garland, Michael (M); Grabosch, Shannon (S); Kelamis, Joseph (J); Messamore, Will (W); Bradley, Todd (T); Sourk, Allison (A); Westberg, Lindsey (L); Goodnight, Waco (W); Tongson, Krystina (K); Holloway, Naomi (N); Wardak, Zabi (Z); Sudyka, Michelle (M); Masrani, Shriti (S); Chintalapati, Sanket (S); Cagaanan, Manuel (M); Brown, John C (JC); Stetler, Dean A (DA);

Affiliation: Department of Molecular Biosciences, University of Kansas. Lawrence, KS 66045, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Autoimmunity (Autoimmunity), published in England. (Language: eng)

Reference: 2007-Feb; vol 40 (issue 1) : pp 38-47

Dates: Created 2007/03/16; Completed 2007/06/19; Revised 2008/11/21;

PMID: 17364496, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Antibodies, Antinuclear - biosynthesis; immunology
Autoantigens - biosynthesis; immunology
Autoimmunity - immunology
Female
Immunization
Lupus Erythematosus, Systemic - immunology
Male
Mice

Mice, Inbred BALB C
Mice, Inbred MRL lpr
Protein Structure, Tertiary
RNA Polymerase I - immunology
Rabbits
Rats
Ribonucleoproteins - biosynthesis; immunology
Ribonucleoproteins, Small Nuclear - biosynthesis; immunology
snRNP Core Proteins

Associated Chemicals: Antibodies, Antinuclear (0) ; Autoantigens (0) ; Ribonucleoproteins (0) ; Ribonucleoproteins, Small Nuclear (0) ; SS-B antigen (0) ; snRNP Core Proteins (0) ; RNA Polymerase I (EC 2.7.7.-)

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