Research article summary (published 30 Dec 2006):

Effects of celecoxib on migration, proliferation and collagen expression of tendon cells.

Full Abstract

Sports-related tendinopathy is commonly treated with nonsteroidal anti-inflammatory drugs including cyclooxygenase-2 inhibitor. Tendon healing requires migration of tendon cells to the repair site, followed by proliferation and synthesis of collagen. This study was designed to determine the effects of COX-2 inhibitor (celecoxib) on the migration, proliferation, and types I and III collagen expression of tendon cells intrinsic to rat Achilles tendon. Using cultured tendon cells, cell migration and proliferation were evaluated by transwell filter migration assay and by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay, respectively. The mRNA expression of alpha1(I) procollagen and alpha1(III) procollagen were determined by reverse transcription-polymerase chain reaction (RT-PCR). The protein expression of types I and III collagen were determined by immunocytochemistry. Dose-dependent celecoxib inhibition was demonstrated for migration of tendon cells through the transwell filter migration assay (p=0.002). Dose-dependent celecoxib inhibition of tendon cell proliferation also was demonstrated by MTT assay (p=0.004). However, both RT-PCR and immunocytochemical staining revealed that mRNA and protein expression of types I and III collagen remained constant after celecoxib treatment. In conclusion, celecoxib inhibits tendon cell migration and proliferation. However, the expression of types I and III collagen remained unchanged.

Author information

Author/s: Tsai, Wen-Chung (WC); Hsu, Chih-Chin (CC); Chou, Shih-Wei (SW); Chung, Chia-Ying (CY); Chen, Jannie (J); Pang, Jong-Hwei S (JH);

Affiliation: Department of Physical Medicine and Rehabilitation, Chang Gung Memorial Hospital, College of Medicine, Chang Gung University, Taoyuan, Taiwan. tsaiwc(-atsign-)adm.cgmh.org.tw

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Connective tissue research (Connect Tissue Res), published in United States. (Language: eng)

Reference: 2007-; vol 48 (issue 1) : pp 46-51

Dates: Created 2007/03/16; Completed 2007/04/27;

PMID: 17364667, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Achilles Tendon - cytology; drug effects; injuries Animals Cell Movement - drug effects Cell Proliferation - drug effects Cells, Cultured Collagen Type I - biosynthesis; drug effects

Collagen Type III - biosynthesis; drug effects Cyclooxygenase 2 Inhibitors - pharmacology Pyrazoles - pharmacology Rats Sulfonamides - pharmacology Wound Healing - drug effects

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; Cyclooxygenase 2 Inhibitors (0) ; Pyrazoles (0) ; Sulfonamides (0) ; celecoxib (169590-42-5)

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