Determination of medroxyprogesterone acetate residues by CE immunoassay with chemiluminescence detection.

Full Abstract

A rapid and simple method is developed for the determination of medroxyprogesterone acetate (MPA) by CE immunoassay with chemiluminescence (CL). This method is based on the competitive reactions between horseradish peroxidase (HRP)-labeled MPA (MPA-HRP) and free MPA with anti-MPA antiserum. The influencing factors on the electrophoresis and CL detection were studied completely and the optimal conditions of separation and determination were obtained. The linear range was 2.0-50 nmol/L and the LOD for MPA was 0.9 nmol/L. The present method was applied to the analysis of pork tissues.

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Author information

Author/s: Peng, Chi-Fang (CF); Huo, Tie-Ming (TM); Liu, Li-Qiang (LQ); Chu, Xiao-Gang (XG); Xu, Chuan-Lai (CL);

Affiliation: School of Food Science and Technology, Southern Yangtze University, WuXi, JiangSu Province, PR China.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article

Journal: Electrophoresis (Electrophoresis), published in Germany. (Language: eng)

Reference: 2007-Mar; vol 28 (issue 6) : pp 970-4

Dates: Created 2007/05/03; Completed 2007/07/30;

PMID: 17370306, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Chemiluminescent Measurements
Electrophoresis, Capillary - methods
Immunoassay - methods

Immunoassay - methods
Lod Score
Medroxyprogesterone 17-Acetate - analysis
Sensitivity and Specificity

Associated Chemicals: Medroxyprogesterone 17-Acetate (71-58-9)

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