Analysis of phospholipids by NACE with on-line ESI-MS.

Full Abstract

A hyphenated method of nonaqueous capillary electrophoresis coupled to electrospray ionization mass spectrometry (NACE-ESI-MS) is described for the simultaneous analysis of phospholipids. The best results were obtained with a mixed solution of methanol/ACN (40:60 v/v) containing 20 mM ammonium acetate and 0.5% acetic acid, under the applied voltage of 30 kV and capillary temperature of 25 degrees C. ESI-MS measurements were performed in the negative mode with methanol/ACN (40:60 v/v) containing 50 mM ammonium acetate as sheath liquid at a flow rate of 2 microL/min. Different phospholipid classes have been successfully separated within 16 min, and the molecular species of every single class have been identified by using MS(2) or MS(3), which generates characteristic fragments through CID. The developed method has been applied to analyze the phospholipids extracted from rat peritoneal surface and the molecular species of phospholipid classes are presented.

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Author information

Author/s: Gao, Fei (F); Zhang, Zhengxiang (Z); Fu, Xiaofang (X); Li, Wei (W); Wang, Tao (T); Liu, Huwei (H);

Affiliation: Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Peking University, Beijing, P. R. China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Electrophoresis (Electrophoresis), published in Germany. (Language: eng)

Reference: 2007-May; vol 28 (issue 9) : pp 1418-25

Dates: Created 2007/05/24; Completed 2007/08/09;

PMID: 17372939, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetonitriles - chemistry
Animals
Buffers
Electrophoresis, Capillary - methods
Methanol - chemistry
Phospholipids - analysis; chemistry

Phospholipids - analysis; chemistry
Plant Extracts - analysis
Rats
Reproducibility of Results
Sensitivity and Specificity
Spectrometry, Mass, Electrospray Ionization - methods

Associated Chemicals: Acetonitriles (0) ; Buffers (0) ; Phospholipids (0) ; Plant Extracts (0) ; Methanol (67-56-1) ; acetonitrile (75-05-8)

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