Research article summary (published 21 Dec 2006):

Cannabinoid modulation of neuronal function after oxygen/glucose deprivation in area CA1 of the rat hippocampus.

Full Abstract

Endocannabinoids released during cerebral ischemia have been implicated as neuroprotective agents. We assessed the role of cannabinoid receptors in modulating the response of neurons to oxygen/glucose deprivation (OGD), a model for in vitro ischemia, in rat hippocampal slices using extracellular recording techniques. Under control conditions, 15 min OGD resulted in only 50% recovery of CA1 field excitatory postsynaptic potentials (fEPSPs) 60 min post-insult. This post-OGD depression of function was primarily NMDA receptor-dependent as the NMDA receptor antagonist MK-801 (50 microM) promoted recovery of synaptic transmission to 76% of the baseline. Treatment with the CB1 receptor antagonist AM251 (1 microM), which prevented the depression of excitatory synaptic transmission caused by WIN55,212-2 (1 microM), also markedly enhanced recovery of function (71% of control). The enhanced recovery after OGD in the presence of AM251 was independent of both GABA(A) receptors and NMDA receptors since co-application of AM251 with either bicuculline (10 microM) or MK-801 (50 microM) did not alter recovery, or further improved recovery, respectively. These results suggest endocannabinoids released during OGD may modulate synaptic transmission and post-OGD neuronal outcome via activation of an AM251-sensitive cannabinoid receptor.

Author information

Author/s: Youssef, Farid F (FF); Hormuzdi, Sheriar G (SG); Irving, Andrew J (AJ); Frenguelli, Bruno G (BG);

Affiliation: Department of Preclinical Sciences, Faculty of Medical Sciences, The University of the West Indies, St. Augustine, Trinidad and Tobago, W.I. fmyoussef(-atsign-)tstt.net.tt

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't

Journal: Neuropharmacology (Neuropharmacology), published in England. (Language: eng)

Reference: 2007-May; vol 52 (issue 6) : pp 1327-35

Dates: Created 2007/04/23; Completed 2007/07/23;

PMID: 17382973, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Benzoxazines - pharmacology Cannabinoids - pharmacology Dizocilpine Maleate - pharmacology Excitatory Amino Acid Antagonists - pharmacology Excitatory Postsynaptic Potentials - drug effects Extracellular Space - drug effects; metabolism; physiology Female Glucose - deficiency Hippocampus - drug effects; physiopathology Hypoxia, Brain - physiopathology

Male Morpholines - pharmacology Naphthalenes - pharmacology Neurons - drug effects; physiology Piperidines - pharmacology Pyrazoles - pharmacology Rats Rats, Sprague-Dawley Receptor, Cannabinoid, CB1 - agonists; antagonists & inhibitors Receptors, N-Methyl-D-Aspartate - antagonists & inhibitors Synaptic Transmission - drug effects

Associated Chemicals: AM 251 (0) ; Benzoxazines (0) ; Cannabinoids (0) ; Excitatory Amino Acid Antagonists (0) ; Morpholines (0) ; Naphthalenes (0) ; Piperidines (0) ; Pyrazoles (0) ; Receptor, Cannabinoid, CB1 (0) ; Receptors, N-Methyl-D-Aspartate (0) ; Win 55212-2 (134959-51-6) ; Glucose (50-99-7) ; Dizocilpine Maleate (77086-22-7)

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