Method development for the determination of coumarin compounds by capillary electrophoresis with indirect laser-induced fluorescence detection.

Full Abstract

A capillary zone electrophoresis (CZE) with indirect laser-induced fluorescence detection (ILIFD) method is described for the simultaneous determination of esculin, esculetin, isofraxidin, genistein, naringin and sophoricoside. The baseline separation was achieved within 5 min with running buffer (pH 9.4) composed of 5mM borate, 20% methanol (v/v) as organic modifier, 10(-7)M fluorescein sodium as background fluorophore and 20 kV of applied voltage at 30 degrees C of cartridge temperature. Good linearity relationships (correlation coefficients >0.9900) between the second-order derivative peak-heights (RFU) and concentrations of the analytes (mol L(-1)) were obtained. The detection limits for all analytes in second-order derivative electrophoregrams were in the range of 3.8-15 microM. The RSD data of intra-day for migration times and second-order derivative peak-height were less than 0.95 and 5.02%, respectively. This developed method was applied to the analysis of the courmin compounds in herb plants with recoveries in the range of 94.7-102.1%. In this work, although the detection sensitivity was lower than that of direct LIF, yet the method would extend the application range of LIF detection.

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Author information

Author/s: Wang, Weiping (W); Tang, Jianghong (J); Wang, Shumin (S); Zhou, Lei (L); Hu, Zhide (Z);

Affiliation: Department of Chemistry, Lanzhou University, Lanzhou 730000, China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2007-Apr; vol 1148 (issue 1) : pp 108-14

Dates: Created 2007/04/04; Completed 2007/06/11;

PMID: 17383667, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Benzopyrans - analysis; chemistry
Coumarins - analysis; chemistry
Electrophoresis, Capillary - methods
Esculin - analysis; chemistry
Flavanones - analysis; chemistry
Genistein - analysis; chemistry

Genistein - analysis; chemistry
Lasers
Molecular Structure
Reproducibility of Results
Spectrometry, Fluorescence - methods
Umbelliferones - analysis; chemistry

Associated Chemicals: Benzopyrans (0) ; Coumarins (0) ; Flavanones (0) ; Umbelliferones (0) ; sophoricoside (0) ; naringin (10236-47-2) ; esculetin (305-01-1) ; Genistein (446-72-0) ; isofraxidin (486-21-5) ; Esculin (531-75-9)

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