Comparison of intranasal with targeted lymph node immunization using PR8-Flu ISCOM adjuvanted HIV antigens in macaques.

Full Abstract

The rapidly spreading HIV epidemic requires a vaccine that elicits potent mucosal immunity to halt or slow transmission. Induction of these responses will depend on the use of appropriate adjuvants and targeting of the mucosal immune system. Previously, immune stimulating complexes (ISCOM) have shown great potency as adjuvant in the induction of mucosal responses in mice and systemic responses in non-human primates. In this study, HIV formulated in PR8-Flu ISCOM adjuvant was applied to immunize rhesus macaques against HIV; targeting the mucosa either via intranasal (IN) application or via targeted lymph node immunization (TLNI). While, strong systemic, HIV specific, cytokine, lymphoproliferative, and antibody responses were induced via the TLNI route, the IN application generated only low responses. Furthermore, all four animals immunized via TLNI developed vaginal IgA antibodies against gp120. In conclusion, in contrast to what has been demonstrated in mice, the IN application of PR8-Flu ISCOM did not induce strong immune responses in rhesus macaques unlike those immunized by the TLNI route.

Learn Faster Today Improve your study skills

Author information

Author/s: Koopman, G (G); Bogers, W M J M (WM); van Gils, M (M); Koornstra, W (W); Barnett, S (S); Morein, B (B); Lehner, T (T); Heeney, J L (JL);

Affiliation: Department of Virology, Biomedical Primate Research Centre, Rijswijk, The Netherlands. koopman(-atsign-)bprc.nl

Grants: 1P01 AI48225-01A2 (Agency:NIAID NIH HHS)

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Journal of medical virology (J Med Virol), published in United States. (Language: eng)

Reference: 2007-May; vol 79 (issue 5) : pp 474-82

Dates: Created 2007/04/02; Completed 2007/06/26; Revised 2007/12/03;

PMID: 17385685, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

AIDS Vaccines - administration & dosage; immunology
Adjuvants, Immunologic - administration & dosage
Administration, Intranasal
Animals
Antibody Specificity
Female
HIV Antibodies - analysis
HIV Core Protein p24 - administration & dosage; immunology
HIV Envelope Protein gp120 - administration & dosage; immunology
HIV Infections - immunology

HIV-1 - immunology
Humans
ISCOMs - administration & dosage
Immunization
Immunization Schedule
Immunoglobulin A - analysis
Injections, Intralymphatic
Macaca mulatta
Vaccines, Subunit - administration & dosage
Vagina - immunology

Associated Chemicals: AIDS Vaccines (0) ; Adjuvants, Immunologic (0) ; HIV Antibodies (0) ; HIV Core Protein p24 (0) ; HIV Envelope Protein gp120 (0) ; ISCOMs (0) ; Immunoglobulin A (0) ; Vaccines, Subunit (0)

These are the highest related articles currently in the database:

See 100+ related articles.