A unifying concept of trophoblastic differentiation and malignancy defined by biomarker expression.

Full Abstract

Several trophoblast phenotypes, including cytotrophoblast, syncytiotrophoblast, and extravillous trophoblast, emerge during gestation. To clarify the lineage relationship between these subtypes, we profiled p63 localization in developing and term placental tissue, as well as in trophoblastic tumors, using antibodies specific to full-length (TAp63) and one against all p63 isoforms (TAp63 and DeltaNp63). Localization of p63 was compared with that of biomarkers of proliferation and trophoblastic differentiation, including mib-1, inhibin, and MelCAM. In early gestation, p63 was localized principally to villous cytotrophoblast after contact with the villous mesenchyme, absent in the trophoblast columns, and early implantation trophoblast. In the maturing placenta, intraplacental perivillous fibrin correlated with the emergence of a p63-positive "transitional" (vacuolated) extravillous trophoblast from cytotrophoblast, which differentiated further into a "mature" p63-negative extravillous trophoblast. The same lineage pathway emerged from entrapped villi on the chorionic membrane. Virtually all p63 immunopositivity was attributed to dominant-negative p63. The immunophenotypic patterns seen in the immature and mature placenta permit the resolution of all trophoblastic phenotypes within 3 lineage pathways of cytotrophoblast differentiation, including cytotrophoblast-to-trophoblast column/implantation site, cytotrophoblast-to-syncytiotrophoblast, and cytotrophoblast-to-mature extravillous trophoblast. In the latter pathway, a transitional (vacuolated) p63-positive extravillous trophoblast emerges from and links cytotrophoblast to mature extravillous trophoblast in intraplacental fibrin, chorionic membrane, and basal plate. The placental trophoblast is thus resolved within this continuum of differentiation. Terms such as transitional and mature extravillous trophoblast are proposed to reflect the differentiation phases of this unique epithelium. p63 staining patterns in trophoblastic tumors reflect timing of neoplastic transformation during trophoblastic differentiation.

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Author information

Author/s: Lee, Yonghee (Y); Kim, Kyu-Rae (KR); McKeon, Frank (F); Yang, Annie (A); Boyd, Theonia K (TK); Crum, Christopher P (CP); Parast, Mana M (MM);

Affiliation: Division of Women's and Perinatal Pathology, Department of Pathology, Brigham and Women's Hospital, Boston, MA 02115, USA.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Human pathology (Hum Pathol), published in United States. (Language: eng)

Reference: 2007-Jul; vol 38 (issue 7) : pp 1003-13

Dates: Created 2007/06/18; Completed 2007/08/03;

PMID: 17397906, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Antigens, CD146 - analysis
Biological Markers - analysis
Female
Humans
Immunohistochemistry
Inhibins - analysis

Inhibins - analysis
Membrane Proteins - analysis
Pregnancy
Trophoblastic Neoplasms - metabolism
Trophoblasts - metabolism
Ubiquitin-Protein Ligases - analysis

Associated Chemicals: Antigens, CD146 (0) ; Biological Markers (0) ; CKAP4 protein, human (0) ; Membrane Proteins (0) ; Inhibins (57285-09-3) ; MIB1 protein, human (EC 6.3.2.19) ; Ubiquitin-Protein Ligases (EC 6.3.2.19)

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