REKLES is an ARID3-restricted multifunctional domain.

Full Abstract

Bright/Dril1/ARID3a is a B cell-specific, matrix association (or attachment) region-binding transcriptional regulator of immunoglobulin heavy chain genes and of E2F1-dependent cell cycle progression. Bright contains a central DNA binding domain termed ARID (AT-rich interacting domain) and a C-terminal region termed REKLES (for a conserved amino acid motif). The ARID domain has been identified in seven highly conserved families of metazoan proteins (ARID1-5 and JARID1-2), whereas REKLES is found only in the ARID3 subfamily (composed of Bright/ARID3a, Bdp/ARID3b, and Bright-like/ARID3c). REKLES consists of two subdomains:
a modestly conserved N-terminal REKLESalpha and a highly conserved (among ARID3 orthologous proteins) C-terminal REKLESbeta. Previously we showed that Bright undergoes nucleocytoplasmic shuttling and that REKLESalpha and -beta were required, respectively, for nuclear import and Crm1-dependent nuclear export. Here we show that Bright further requires REKLESbeta for self-association or paralogue association and for nuclear matrix targeting. REK-LES promotes and regulates the extent of Bright multimerization, which occurs in the absence or presence of target DNA and is necessary for specific DNA binding. REKLESbeta-mediated interaction of Bright with Bdp, which localizes strictly to the nucleus, traps Bright within the nucleus via neutralization of its nuclear export activity. These results identify REKLES as a multifunctional domain that has co-evolved with and regulates functional properties of the ARID3 DNA binding domain.

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Author information

Author/s: Kim, Dongkyoon (D); Probst, Loren (L); Das, Chhaya (C); Tucker, Philip W (PW);

Affiliation: Section of Molecular Genetics and Microbiology and Institute of Cell and Molecular Biology, University of Texas, Austin, Texas 78712-0162, USA.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: The Journal of biological chemistry (J Biol Chem), published in United States. (Language: eng)

Reference: 2007-May; vol 282 (issue 21) : pp 15768-77

Dates: Created 2007/05/21; Completed 2007/07/18;

PMID: 17400556, status: MEDLINE (last retrieval date: 12/26/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Active Transport, Cell Nucleus - physiology
Amino Acid Motifs - genetics
Animals
B-Lymphocytes - metabolism
COS Cells
Cercopithecus aethiops
DNA-Binding Proteins - genetics; metabolism
E2F1 Transcription Factor - genetics; metabolism
Evolution, Molecular
Humans

Humans
Immunoglobulin Heavy Chains - biosynthesis; genetics
Nuclear Matrix - genetics; metabolism
Oncogenes - genetics
Organ Specificity - physiology
Protein Structure, Tertiary - genetics
Protozoa - genetics; metabolism
Protozoan Proteins - genetics; metabolism
Sequence Homology, Amino Acid
Trans-Activators - genetics; metabolism

Associated Chemicals: ARID3A protein, human (0) ; DNA-Binding Proteins (0) ; E2F1 Transcription Factor (0) ; E2F1 protein, human (0) ; Immunoglobulin Heavy Chains (0) ; Protozoan Proteins (0) ; Trans-Activators (0)

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