Protein identification using mass spectrometry: a method overview.

Full Abstract

With the introduction of soft ionization techniques such as Matrix Assisted Laser Desorption Ionization (MALDI), and Electrospray Ionization (ESI), proteins have become accessible to mass spectrometric analyses. Since then, mass spectrometry has become the method of choice for sensitive, reliable and inexpensive protein and peptide identification. With the increasing number of full genome sequences for a variety of organisms and the numerous protein databases constructed thereof, all the tools necessary for the high-throughput protein identification with mass spectrometry are in place. This chapter highlights the different mass spectrometric techniques currently applied in proteome research by giving a brief overview of methods for identification of posttranslational modifications and discussing their suitability of strategies for automated data analysis.

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Author information

Author/s: Schuchardt, Sven (S); Sickmann, Albert (A);

Affiliation: Fraunhofer Institute of Toxicology and Experimental Medicine, Drug Research and Medical Biotechnology, Nikolai-Fuchs-Strasse 1, 30625 Hannover, Germany. sven.schuchardt(-atsign-)item.fraunhofer.de

Journal and publication information

Publication Type: Journal Article; Review

Journal: EXS (EXS), published in Switzerland. (Language: eng)

Reference: 2007-; vol 97 (issue ) : pp 141-70

Dates: Created 2007/04/13; Completed 2007/05/02;

PMID: 17432267, status: MEDLINE (last retrieval date: 12/26/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Mass Spectrometry
Peptides - analysis; chemistry
Phosphoproteins - analysis

Protein Processing, Post-Translational
Proteins - analysis; chemistry
Sequence Analysis, Protein

Associated Chemicals: Peptides (0) ; Phosphoproteins (0) ; Proteins (0)

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