[Effects of nuclear factor-kappaB decoy oligodeoxynucleotide on the function of human umbilical artery smooth muscle cells induced by umbilical sera in preeclampsia]

Full Abstract

OBJECTIVE: To study the effects of nuclear factor-kappaB (NF-kappaB) decoy oligodeoxynucleotide (ODN) on the preeclamptic umbilical serum induced expression of precollagen I, III mRNA and tumor necrosis factor-alpha (TNF-alpha) in cultured human umbilical artery smooth muscle cells (HUASMC). METHODS: Primary cultured HUASMC of normal pregnancy were divided into four groups: group A (HUASMC were incubated with umbilical serum of normal pregnancy); group B (HUASMC were incubated with umbilical serum of preeclampsia); group C (HUASMC were transfected with NF-kappaB cis decoy ODN 48 h before incubation with umbilical serum of preeclampsia); group D (HUASMC were transfected with NF-kappaB scramble ODN 24 h before incubation with umbilical serum of preeclampsia). NF-kappaB cis decoy ODN and NF-kappaB scramble ODN were transfected with cationic lipofectamine to the latter two groups, respectively. The proliferation of human umbilical artery smooth muscle cells was evaluated by methyl thiazolyl tetrazolium and the apoptosis was analyzed by flow cytometry. The expression levels of precollagen I, III mRNA were detected by RT-PCR, the expression levels of TNF-alpha were detected by western blot. RESULTS: (1) The proliferation of group B (0.19 +/- 0.02) and group D (0.18 +/- 0.03) was significantly increased as compared with those of group A (0.11 +/- 0.02) and group C (0.14 +/- 0.02) (P < 0.05). (2) The apoptosis rates of group B [(7.8 +/- 1.3)%], group C [(10.1 +/- 1.2)%] and group D [(8.1 +/- 1.3)%] were significantly reduced as compared with that of group A [(14.3 +/- 1.2)%] (P < 0.05), and there was a significant difference between groups B and C (P < 0.05). (3) The expression levels of precollagen I mRNA of group B (0.31 +/- 0.04), group C (0.23 +/- 0.04) and group D (0.30 +/- 0.03) were significantly increased as compared with that of group A (0.16 +/- 0.02) (P < 0.05), and there was a significant difference between groups B and C (P < 0.05). (4) There were no significant differences among the four groups in the expression level of precollagen III mRNA (P > 0.05). (5) The expression of TNF-alpha of group B (0.74 +/- 0.11), group C (0.36 +/- 0.09) and group D (0.79 +/- 0.12) were significantly higher than that of group A (0.15 +/- 0.03) (P < 0.05), and the expression of TNF-alpha of groups B and D were significantly higher than that of group C (P < 0.05), while there was no significant difference between groups B and D (P > 0.05). CONCLUSIONS: NF-kappaB cis decoy ODN could down-regulate the proliferation, as well as the expression levels of precollagen and TNF-alpha of HUASMC induced by umbilical serum of preeclampsia. NF-kappaB may play an important role in the pathogenesis of placental artery abnormalities in preeclampsia.

Author information

Author/s: Jiang, Rong-Zhen (RZ); Huang, Ya-Juan (YJ); Gu, Jing-Hong (JH); Mu, Wei-Hong (WH); Chen, Han-Ping (HP);

Affiliation: Department of Obstetrics and Gynecology, Affiliated Sixth People's Hospital, Shanghai Jiao Tong University, Shanghai 200233, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi), published in China. (Language: chi)

Reference: 2007-Feb; vol 42 (issue 2) : pp 87-91

Dates: Created 2007/04/19; Completed 2007/12/06;

PMID: 17442180, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: 18 Feb 2009 00:00:00)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult
Cell Proliferation - drug effects
Cells, Cultured
Collagen Type I - biosynthesis; genetics
Collagen Type III - biosynthesis; genetics
Female
Fetal Blood
Humans
Muscle, Smooth, Vascular - cytology; drug effects; metabolism

Myocytes, Smooth Muscle - drug effects; metabolism
NF-kappa B - metabolism
Oligonucleotides - pharmacology
Pre-Eclampsia - metabolism; pathology
Pregnancy
RNA, Messenger - biosynthesis
Reverse Transcriptase Polymerase Chain Reaction
Tumor Necrosis Factors - biosynthesis
Umbilical Arteries - cytology; metabolism

Associated Chemicals: Collagen Type I (0) ; Collagen Type III (0) ; NF-kappa B (0) ; Oligonucleotides (0) ; RNA, Messenger (0) ; Tumor Necrosis Factors (0)

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