Research article summary (published 29 Nov 2006):

Osteogenic differentiation of human mesenchymal stem cells in collagen matrices: effect of uniaxial cyclic tensile strain on bone morphogenetic protein (BMP-2) mRNA expression.

Full Abstract

Human mesenchymal stem cells (hMSCs) differentiate down an osteogenic pathway with appropriate mechanical and/or chemical stimuli. This study describes the successful culture of hMSCs in 3D collagen matrices under mechanical strain. Bone marrow-derived hMSCs were seeded in linear 3D type I collagen matrices and subjected to 0%, 10%, or 12% uniaxial cyclic tensile strain at 1 Hz for 4 h/day for 7 or 14 days. Cell viability studies indicated that hMSCs remained viable throughout the culture period irrespective of the applied strain level. Real-time RT-PCR studies indicated a significant increase in BMP-2 mRNA expression levels in hMSCs strained at 10% compared to the same day unstrained controls after both 7 and 14 days. An increase in BMP-2 was also observed in hMSCs subjected to 12% strain, but the increase was significant only in the 14-day sample. This is the first report of the culture of bone marrow-derived hMSCs in 3D collagen matrices under cyclic strain, and the first demonstration that strain alone can induce osteogenic differentiation without the addition of osteogenic supplements. Induction of bone differentiation in 3D culture is a critical step in the creation of bioengineered bone constructs.

Author information

Author/s: Sumanasinghe, Ruwan D (RD); Bernacki, Susan H (SH); Loboa, Elizabeth G (EG);

Affiliation: Joint Department of Biomedical Engineering, University of North Carolina at Chapel Hill and North Carolina State University, Raleigh, North Carolina 27695-7115, USA.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Tissue engineering (Tissue Eng), published in United States. (Language: eng)

Reference: 2006-Dec; vol 12 (issue 12) : pp 3459-65

Dates: Created 2007/05/23; Completed 2007/07/09; Revised 2008/11/21;

PMID: 17518682, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - biosynthesis; genetics Bone and Bones - cytology; metabolism Cell Differentiation - physiology Cell Survival - physiology Cells, Cultured Collagen Type I

Collagen Type I Gene Expression Regulation, Developmental - physiology Humans Male Mesenchymal Stem Cells - cytology; metabolism RNA, Messenger - biosynthesis Tensile Strength Transforming Growth Factor beta - biosynthesis; genetics

Associated Chemicals: BMP2 protein, human (0) ; Bone Morphogenetic Protein 2 (0) ; Bone Morphogenetic Proteins (0) ; Collagen Type I (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta (0)

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