Research article summary (published 27 Feb 2007):

[Homocysteine decreases the invasion in cultured human trophoblasts: relationship between homocysteine and matrix metalloproteinase-2, -9 expression]

Full Abstract

OBJECTIVE: To test the hypothesis that homocysteine can decrease MMP-2 and MMP-9 expression in cultured trophoblasts of early pregnancy and that homocysteine can prevent trophoblasts invasion in the early stage of preeclampsia. METHODS: Cytotrophoblasts from early pregnancy were isolated and cultured. Trophoblasts were treated with or without Hcy (1 mmol/L) for 48 hour, and real time RT-PCR and gelatin zymography were used to quantify the mRNA and protease activity of MMP-2, -9. RESULTS: Treatment with Hcy (1 mmol/L) induced a decrease in MMP-2 mRNA by 21% and MMP-9 mRNA by 11%. At protein level MMP-2 expression decreased 14% and MMP-9 expression decreased 52% compared with control. CONCLUSIONS: Homocysteine can decrease MMP-2, -9 expression in trophoblasts of early pregnancy and influence its invasion process.

Author information

Author/s: Yang, Pei-feng (PF); Li, Jin-ke (JK); Xiong, Qing (Q);

Affiliation: Department of Obstetrics and Gynecology, West China Second Hospital, Sichuan University, Chengdu 610041, China.

Journal and publication information

Publication Type: English Abstract; Journal Article

Journal: Zhonghua fu chan ke za zhi (Zhonghua Fu Chan Ke Za Zhi), published in China. (Language: chi)

Reference: 2007-Mar; vol 42 (issue 3) : pp 184-6

Dates: Created 2007/05/31; Completed 2007/12/11;

PMID: 17537305, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Adult Cells, Cultured Female Gestational Age Homocysteine - pharmacology Humans Matrix Metalloproteinase 2 - biosynthesis; genetics

Matrix Metalloproteinase 9 - biosynthesis; genetics Pre-Eclampsia - metabolism; pathology Pregnancy Pregnancy Trimester, First RNA, Messenger - biosynthesis Reverse Transcriptase Polymerase Chain Reaction Trophoblasts - cytology; drug effects; metabolism

Associated Chemicals: RNA, Messenger (0) ; Homocysteine (454-28-4) ; Matrix Metalloproteinase 2 (EC 3.4.24.24) ; Matrix Metalloproteinase 9 (EC 3.4.24.35)

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