Analyses of tobacco alkaloids by cation-selective exhaustive injection sweeping microemulsion electrokinetic chromatography.

Full Abstract

In this study, an on-line concentration method which coupled cation-selective exhaustive injection (CSEI) sweeping technology with microemulsion electrokinetic chromatography (MEEKC) was used to detect and analyze several tobacco alkaloids (nornicotine, anabasine, anatabine, nicotine, myosmine and cotinine) that are commonly found in various tobacco products. First, the effects of microemulsion compositions (oil, cosurfactant and solution pH) were examined in order to optimize the alkaloid separations in conventional MEEKC. The pH value and the injection length of basic plug were found to be the predominant influences on the alkaloid stacking. This optimal CSEI sweeping MEEKC method provided approximately 180- to 540-fold increase in detection sensitivity in terms of peak height without any loss in separation efficiency when compared to normal MEEKC separation. Furthermore, this proposed CSEI sweeping MEEKC method was applied successfully for the detection of the minor alkaloids nornicotine, anabasine and anatabine in tobacco products.

Author information

Author/s: Huang, Hsi-Ya (HY); Hsieh, Shih-Huan (SH);

Affiliation: R&D Center for Membrane Technology, Department of Chemistry and Center for Nanotechnology, Chung Yuan Christian University, Chung-Li, Taiwan. hyhuang(-atsign-)cycu.edu.tw

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of chromatography. A (J Chromatogr A), published in Netherlands. (Language: eng)

Reference: 2007-Sep; vol 1164 (issue 1-2) : pp 313-9

Dates: Created 2007/08/27; Completed 2007/11/06; Revised 2009/01/15;

PMID: 17644105, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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