Research article summary (published 23 Jul 2007):

Glycosaminoglycan and growth factor mediated murine calvarial cell proliferation.

Full Abstract

Understanding the complex mechanisms underlying bone remodeling is crucial to the development of novel therapeutics. Glycosaminoglycans (GAGs) localised to the extracellular matrix (ECM) of bone are thought to play a key role in mediating aspects of bone development. The influence of isolated GAGs was studied by utilising in vitro murine calvarial monolayer and organ culture model systems. Addition of GAG preparations extracted from the cell surface of human osteoblasts at high concentrations (5 microg/ml) resulted in decreased proliferation of cells and decreased suture width and number of bone lining cells in calvarial sections. When we investigated potential interactions between the growth factors fibroblast growth factor-2 (FGF2), bone morphogenic protein-2 (BMP2) and transforming growth factor-beta1 (TGFbeta1) and the isolated cell surface GAGs, differences between the two model systems emerged. The cell culture system demonstrated a potentiating role for the isolated GAGs in the inhibition of FGF2 and TGFbeta1 actions. In contrast, the organ culture system demonstrated an enhanced stimulation of TFGbeta1 effects. These results emphasise the role of the ECM in mediating the interactions between GAGs and growth factors during bone development and suggest the GAG preparations contain potent inhibitory or stimulatory components able to mediate growth factor activity.

Author information

Author/s: Manton, Kerry J (KJ); Haupt, Larisa M (LM); Vengadasalam, Kumeri (K); Nurcombe, Victor (V); Cool, Simon M (SM);

Affiliation: Stem Cells and Tissue Repair Group, Institute of Molecular and Cell Biology, Proteos, Singapore, Singapore.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of molecular histology (J Mol Histol), published in Netherlands. (Language: eng)

Reference: 2007-Oct; vol 38 (issue 5) : pp 415-24

Dates: Created 2007/11/05; Completed 2008/03/17; Revised 2008/11/21;

PMID: 17653826, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - pharmacology Cell Differentiation - drug effects Cell Proliferation - drug effects Cells, Cultured Dose-Response Relationship, Drug Fibroblast Growth Factor 2 - pharmacology Glycosaminoglycans - pharmacology Humans

Humans Intercellular Signaling Peptides and Proteins - pharmacology Mice Mice, Inbred BALB C Mice, Inbred C57BL Organ Culture Techniques Simvastatin - pharmacology Skull - cytology Transforming Growth Factor beta - pharmacology Transforming Growth Factor beta1 - pharmacology

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: BMP2 protein, human (0) ; Bmp2 protein, mouse (0) ; Bone Morphogenetic Protein 2 (0) ; Bone Morphogenetic Proteins (0) ; Glycosaminoglycans (0) ; Intercellular Signaling Peptides and Proteins (0) ; Transforming Growth Factor beta (0) ; Transforming Growth Factor beta1 (0) ; Fibroblast Growth Factor 2 (103107-01-3) ; Simvastatin (79902-63-9)

Related articles

These are the most related articles currently in our database:

• Proliferation, osteogenic differentiation, and fgf-2 modulation of posterofrontal/sagittal suture-derived mesenchymal cells in vitro. 29 Jun 2008
• Human cardiovascular progenitor cells develop from a KDR+ embryonic-stem-cell-derived population. 21 Apr 2008
• Astroglial-conditioned media and growth factors modulate proliferation and differentiation of astrocytes in primary culture. 5 Dec 2006
• RPE-derived factors modulate photoreceptor differentiation: a possible role in the retinal stem cell niche. 7 Oct 2007
• The activin receptor-like kinase 6 Booroola mutation enhances suppressive effects of bone morphogenetic protein 2 (BMP2), BMP4, BMP6 and growth and differentiation factor-9 on FSH release from ovine primary pituitary cell cultures. 30 Jan 2008
• Pretreatment of adult bone marrow mesenchymal stem cells with cardiomyogenic growth factors and repair of the chronically infarcted myocardium. 18 Oct 2006
• Molecular basis of oocyte-paracrine signalling that promotes granulosa cell proliferation. 20 Aug 2006
• [Osteogenic growth peptide regulates proliferation and differentiation of calvarial osteoblast-like cells of rat] 29 Apr 2006
• Differentiation of autonomic neurons by BMP-independent mechanisms. 13 Jan 2008
• Response of human chondrocytes prepared for autologous implantation to growth factors. 29 Jun 2008

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a larger map of 100+ related articles.