One-step triplex-polymerase chain reaction assay for the authentication of yellowfin (Thunnus albacares), bigeye (Thunnus obesus), and skipjack (Katsuwonus pelamis) tuna DNA from fresh, frozen, and canned tuna samples.

Full Abstract

A one-step triplex-polymerase chain reaction (PCR)-based assay was developed to discriminate between three tuna species, Thunnus albacares, Thunnus obesus, and Katsuwonus pelamis, even in highly processed food samples such as canned or cooked tuna. Diagnostic nucleotides were identified by direct sequencing and alignment of part of the mitochondrial cytochrome b gene of 30 authenticated exemplars, which allowed us to evaluate intraspecific variation and the genetic distance between three tuna species. The assay relies on a one-step triplex-PCR reaction in which in a single tube species-specific amplification products are generated only in the presence of the correct template nucleic acid and the species of origin of the DNA is indicated by the distinctive size of the PCR product. The identification of tuna species can be performed with a good accuracy, low cost, and with potential automation for large-scale high-throughput screenings in small in-house laboratories.

Author information

Author/s: Michelini, Elisa (E); Cevenini, Luca (L); Mezzanotte, Laura (L); Simoni, Patrizia (P); Baraldini, Mario (M); De Laude, Luca (L); Roda, Aldo (A);

Affiliation: Department of Pharmaceutical Sciences, via Belmeloro 6, Bologna, Italy.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)

Reference: 2007-Sep; vol 55 (issue 19) : pp 7638-47

Dates: Created 2007/09/13; Completed 2007/12/12;

PMID: 17711337, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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