Research article summary (published 13 Sep 2007):

Routine forensic use of the mitochondrial 12S ribosomal RNA gene for species identification.

Full Abstract

Since July 2004, Mitotyping Technologies has been amplifying and sequencing a approximately 150 base pair fragment of mitochondrial DNA (mtDNA) that codes for 12S ribosomal RNA, to identify the species origin of nonhuman casework samples. The approximately 100 base pair sequence product is searched at http://www.ncbi.nlm.nih.gov/BLAST and the species match is reported. The use of this assay has halved the number of samples for which no mtDNA results are obtained and is especially useful on hairs and degraded samples. The availability of species determination may aid forensic investigators in opening or closing off lines of inquiry where a highly probative but challenging sample has been collected.

Author information

Author/s: Melton, Terry (T); Holland, Charity (C);

Affiliation: Mitotyping Technologies, 2565 Park Center Boulevard, Suite 200, State College, PA 16801, USA. twm107(-atsign-)mitotyping.com

Journal and publication information

Publication Type: Journal Article

Journal: Journal of forensic sciences (J Forensic Sci), published in United States. (Language: eng)

Reference: 2007-Nov; vol 52 (issue 6) : pp 1305-7

Dates: Created 2007/12/20; Completed 2008/02/28;

PMID: 17868265, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals DNA Fingerprinting - methods DNA Primers DNA, Mitochondrial - genetics Deer - genetics Dipodomys - genetics Dogs - genetics Humans

Humans Mice - genetics Polymerase Chain Reaction Puma - genetics RNA, Ribosomal - genetics Sciuridae - genetics Sequence Analysis, DNA Species Specificity

Associated Chemicals: DNA Primers (0) ; DNA, Mitochondrial (0) ; RNA, Ribosomal (0) ; RNA, ribosomal, 12S (0)

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