Research article summary (published 29 Oct 2007):

Hormonal regulation of Galphai2 and mPRalpha in immortalized human oviductal cell line OE-E6/E7.

Full Abstract

Heterotrimeric G proteins play a key role in membrane-mediated cell-signalling and hormonal regulation. Our earlier studies gave evidence of G protein subunit Galpha(i2) being under hormonal regulation in human in vivo. In this study, we used immortalized human oviduct epithelial cell line OE-E6/E7 as a model to study the hormonal regulation of Galpha(i2). We aimed at clarifying whether estradiol or progesterone could individually regulate the expression of Galpha(i2) and its potential signalling partners. Furthermore, we aimed to investigate which sex hormone receptors could potentially mediate the gene regulation in OE-E6/E7 cell line. OE-E6/E7 cells were cultured for 5 days with different concentrations of estradiol or progesterone. Quantitative real-time polymerase chain reaction (Q-PCR) was performed using cDNA of the hormone-treated cells to reveal any changes in gene expression. The presence of potential receptor targets in these cells was studied using PCR. Our data clearly showed that low concentrations of estradiol up-regulated the expression of Galpha(i2) gene and down-regulated the expression of membrane progesterone receptor mPRalpha gene in OE-E6/E7 cell line. Progesterone had no significant effect on Galpha(i2) gene expression, but it caused up-regulation of mPRalpha gene expression. In conclusion, it appears that sex hormones regulate the expression of Galpha(i2) and mPRalpha genes in a reverse manner in OE-E6/E7 cells. Our results suggest that estrogen receptor ERbeta mediates the regulatory effects of estradiol in these cells.

Author information

Author/s: Mönkkönen, Kati S (KS); Aflatoonian, Reza (R); Lee, Kai-Fai (KF); Yeung, William S B (WS); Tsao, Sai-Wah (SW); Laitinen, Jarmo T (JT); Fazeli, Alireza (A);

Affiliation: Department of Pharmacology and Toxicology, University of Kuopio, FIN-70211 Kuopio, Finland.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Molecular human reproduction (Mol Hum Reprod), published in England. (Language: eng)

Reference: 2007-Dec; vol 13 (issue 12) : pp 845-51

Dates: Created 2007/12/21; Completed 2008/07/15;

PMID: 17977902, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Blotting, Western Cell Line Estradiol - pharmacology Fallopian Tubes - cytology; drug effects; metabolism Female GTP-Binding Protein alpha Subunit, Gi2 - genetics; metabolism

Gene Expression Regulation - drug effects Humans Progesterone - pharmacology Receptors, G-Protein-Coupled - genetics; metabolism Receptors, Progesterone - genetics; metabolism Reverse Transcriptase Polymerase Chain Reaction

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Receptors, G-Protein-Coupled (0) ; Receptors, Progesterone (0) ; membrane progestin receptor alpha, human (0) ; Estradiol (50-28-2) ; Progesterone (57-83-0) ; GTP-Binding Protein alpha Subunit, Gi2 (EC 3.6.1.46)

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