Wireless voltammetry recording in unanesthetised behaving rats.

Full Abstract

In vivo voltammetry is a valuable technique for rapid measurement of dopamine in the brain of freely behaving rats. Using a conventional voltammetry system, however, behavioural freedom is restricted by cables connecting the head assembly to the measurement system. To overcome these difficulties, we developed a wireless voltammetry system utilizing radio waves. This system consisted of a potentiostat and transmitter system that was mounted on the back of the rat, and a receiver and analysis system. A single-step pulse (100-250 mV) was applied at 4 Hz after an activation pulse to a carbon fibre recording electrode (diameter: 7 microm). Measurement of dopamine (detection limit: 2.7 x 10(-7)M) was demonstrated in vitro. In vivo experiment was performed at least 1 week after the recording electrode was implanted in the rat striatum. Administration of 2-phenylethylamine to rats increased dopamine signal current, which was consistent with the result in the microdialysis measurement. During a resident-intruder test, dopamine signal current in a resident rat increased upon introduction of an intruder rat. These results show that the present wireless system is useful for a long-term measurement of dopamine in behaving rats.

Author information

Author/s: Kagohashi, Maki (M); Nakazato, Taizo (T); Yoshimi, Kenji (K); Moizumi, Shunjiro (S); Hattori, Nobutaka (N); Kitazawa, Shigeru (S);

Affiliation: Department of Physiology, Juntendo University School of Medicine, Hongo 2-1-1, Tokyo 113-0033, Japan.

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Neuroscience research (Neurosci Res), published in Ireland. (Language: eng)

Reference: 2008-Jan; vol 60 (issue 1) : pp 120-7

Dates: Created 2008/01/07; Completed 2008/04/11;

PMID: 17983679, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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