Research article summary (published 26 Nov 2007):

Functional compartmentalization of endosomal trafficking for the synaptic delivery of AMPA receptors during long-term potentiation.

Full Abstract

Endosomal membrane trafficking in dendritic spines is important for proper synaptic function and plasticity. However, little is known about the molecular identity and functional compartmentalization of the membrane trafficking machinery operating at the postsynaptic terminal. Here we report that the transport of AMPA-type glutamate receptors into synapses occurs in two discrete steps, and we identify the specific endosomal functions that control this process during long-term potentiation. We found that Rab11-dependent endosomes translocate AMPA receptors from the dendritic shaft into spines. Subsequently, an additional endosomal trafficking step, controlled by Rab8, drives receptor insertion into the synaptic membrane. Separate from this receptor delivery route, we show that Rab4 mediates a constitutive endosomal recycling within the spine. This Rab4-dependent cycling is critical for maintaining spine size but does not influence receptor transport. Therefore, our data reveal a highly compartmentalized endosomal network within the spine and identify the molecular components and functional organization of the membrane organelles that mediate AMPA receptor synaptic delivery during plasticity.

Author information

Author/s: Brown, Tyler C (TC); Correia, Susana S (SS); Petrok, Cortney N (CN); Esteban, José A (JA);

Affiliation: Neuroscience Program and Department of Pharmacology, University of Michigan Medical School, Ann Arbor, Michigan 48109, USA.

Grants: F31-MH070205 (Agency:NIMH NIH HHS) ; MH070417 (Agency:NIMH NIH HHS)

Journal and publication information

Publication Type: In Vitro; Journal Article; Research Support, N.I.H., Extramural

Journal: The Journal of neuroscience : the official journal of the Society for Neuroscience (J Neurosci), published in United States. (Language: eng)

Reference: 2007-Nov; vol 27 (issue 48) : pp 13311-5

Dates: Created 2007/11/29; Completed 2008/01/03;

PMID: 18045925, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Analysis of Variance Animals Calcium-Calmodulin-Dependent Protein Kinase Type 2 - genetics; metabolism Dendrites - metabolism Electric Stimulation - methods Endosomes - metabolism Gynecomastia Hippocampus - cytology; metabolism Long-Term Potentiation - genetics; physiology; radiation effects Luminescent Proteins - genetics; metabolism

Luminescent Proteins - genetics; metabolism Neurons - cytology; physiology Patch-Clamp Techniques - methods Protein Transport - physiology Rats Receptors, AMPA - metabolism Receptors, Glutamate - genetics; metabolism Synaptic Membranes - metabolism Transformation, Genetic rab GTP-Binding Proteins - metabolism

Associated Chemicals: Luminescent Proteins (0) ; Receptors, AMPA (0) ; Receptors, Glutamate (0) ; Calcium-Calmodulin-Dependent Protein Kinase Type 2 (EC 2.7.11.17) ; rab GTP-Binding Proteins (EC 3.6.1.-)

Related articles

These are the highest related articles currently in the database:

• Activation of PI3-kinase is required for AMPA receptor insertion during LTP of mEPSCs in cultured hippocampal neurons. 20 May 2003
• Recycling endosomes supply AMPA receptors for LTP. 22 Sep 2004
• NMDA receptor-dependent long-term potentiation in mouse hippocampal interneurons shows a unique dependence on Ca(2+)/calmodulin-dependent kinases. 18 Sep 2007
• NMDA receptor-mediated Na+ signals in spines and dendrites. 13 Jun 2001
• Regulation of dendritic excitability by activity-dependent trafficking of the A-type K+ channel subunit Kv4.2 in hippocampal neurons. 19 Jun 2007
• Synapses with a segmented, completely partitioned postsynaptic density express more AMPA receptors than other axospinous synaptic junctions. 30 Dec 2003
• Multiple mechanisms for the potentiation of AMPA receptor-mediated transmission by alpha-Ca2+/calmodulin-dependent protein kinase II. 30 May 2002
• Reduced long-term potentiation in the dentate gyrus of mGlu1 receptor-mutant mice in vivo. 20 Apr 1996
• Dynamics of postsynaptic glutamate receptor targeting. 9 Dec 2006
• Sindbis viral-mediated expression of Ca2+-permeable AMPA receptors at hippocampal CA1 synapses and induction of NMDA receptor-independent long-term potentiation. 30 Mar 2001

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.