Research article summary (published 30 Dec 2007):

PCR of immobilized DNA molecules isolated from human blood cells by a non-enzymatic method.

Full Abstract

DNA molecules suitable for amplification by Polymerase Chain Reaction were obtained by immobilizing whole blood or isolated leukocytes and incubating the immobilized cells for one hour with the known non-enzymatic solution described for preparing intact DNA molecules for PFGE. Cell immobilization was done in agarose gels and punches of 1.2 mm of diameter had the amount of DNA needed for amplifying chromosomal and mitochondrial sequences, many times. The approach was successfully used in preparing DNA molecules from multiple samples in flat-bottom 96-well ELISA plates. The procedure is simple and does not demand special conditions for sample transportation or conservation; thus, it should be useful to collect and process samples under field conditions in epidemiological studies.

Author information

Author/s: Suárez, Viana Manrique (VM); Clarke, David Higginson (DH); López-Canovas, Lilia (L); Riverón, Ana María (AM);

Affiliation: Molecular Biology Department, Cuban Neurosciences Center, Havana, Cuba.

Journal and publication information

Publication Type: Journal Article

Journal: Preparative biochemistry & biotechnology (Prep Biochem Biotechnol), published in United States. (Language: eng)

Reference: 2008-; vol 38 (issue 1) : pp 40-50

Dates: Created 2007/12/17; Completed 2008/04/03;

PMID: 18080909, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Base Sequence Biosensing Techniques Blood Cells - chemistry; metabolism Chromosomes, Human - genetics DNA - blood; genetics; isolation & purification DNA, Mitochondrial - genetics

DNA, Mitochondrial - genetics Electrophoresis, Agar Gel Humans Nucleic Acid Amplification Techniques Polymerase Chain Reaction Sensitivity and Specificity

Associated Chemicals: DNA, Mitochondrial (0) ; DNA (9007-49-2)

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