Research article summary (published 11 Dec 2007):

Complete mitochondrial genome sequence of the wine yeast Candida zemplinina: intraspecies distribution of a novel group-IIB1 intron with eubacterial affiliations.

Full Abstract

The mtDNA of the ascomycetous wine yeast Candida zemplinina is a circularly mapping genome of 23,114 bp. It contains 35 genes coding for the seven basic subunits of oxidative phosporylation found in yeasts (the genes encoding for NADH oxidoreductase subunits are absent), the ribosomal protein Var1, two rRNAs and 25 tRNA genes. Although protein phylogenetic analysis showed a divergent mitochondrial genome, several traits appeared preserved. The conserved gene blocks between the mtDNAs of C. zemplinina and Candida glabrata were maintained and changes in gene order and putative promoters were due to restricted genome reshuffling. New heterogeneous hairpin elements were identified scattered throughout cox1 introns. The large subunit rRNA gene harboured the first group-IIB1 intron containing a putative active reverse transcriptase (RT) in mitochondrial genomes of fungi. Phylogenetic analysis of the RT protein confirmed its closer relationship to eubacterial intronic RTs, while being only distantly related to all other fungal mitochondrial group-II introns and RTs. The findings point towards an early migration event of a eubacterial group-II intron to the mitochondrial genome of C. zemplinina.

Author information

Author/s: Pramateftaki, Paraskevi V (PV); Kouvelis, Vassili N (VN); Lanaridis, Panagiotis (P); Typas, Milton A (MA);

Affiliation: Wine Institute of Athens, National Agricultural Research Foundation (N.AG.RE.F.), Lykovrissi, Greece.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: FEMS yeast research (FEMS Yeast Res), published in England. (Language: eng)

Reference: 2008-Mar; vol 8 (issue 2) : pp 311-27

Dates: Created 2008/02/13; Completed 2008/05/05;

PMID: 18081838, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence Base Sequence Candida - genetics DNA, Fungal - chemistry; genetics DNA, Mitochondrial - chemistry; genetics Fungal Proteins - genetics Gene Order Gene Rearrangement - genetics Genes, Fungal Genes, rRNA Genome, Mitochondrial

Introns Models, Molecular Molecular Sequence Data Nucleic Acid Conformation Oxidative Phosphorylation Phylogeny RNA, Transfer - genetics RNA-Directed DNA Polymerase - genetics Ribosomal Proteins - genetics Sequence Analysis, DNA Sequence Homology

Associated Chemicals: DNA, Fungal (0) ; DNA, Mitochondrial (0) ; Fungal Proteins (0) ; Ribosomal Proteins (0) ; RNA, Transfer (9014-25-9) ; RNA-Directed DNA Polymerase (EC 2.7.7.49)

Related articles

These are the highest related articles currently in the database:

• Intraspecific comparison and annotation of two complete mitochondrial genome sequences from the plant pathogenic fungus Mycosphaerella graminicola. 21 Dec 2007
• Comparative analysis of the complete mitochondrial genomes of Aspergillus niger mtDNA type 1a and Aspergillus tubingensis mtDNA type 2b. 30 Mar 2008
• Mitochondrial transfer RNA genes of the yeast Candida parapsilosis. 14 May 1994
• Analysis of a five gene cluster and unique orientation of large genic sequences in Torulopsis glabrata mitochondrial DNA. 30 Dec 1982
• Identification of a Candida utilis gene encoding ribosomal protein L7: evidence for two divergent subclasses of the eukaryotic ribosomal protein L7 family. 23 Jul 1993
• The structure of the small mitochondrial DNA of Kluyveromyces thermotolerans is likely to reflect the ancestral gene order in fungi. 30 May 1994
• Isolation of the ATP synthase subunit 6 and sequence of the mitochondrial ATP6 gene of the yeast Candida parapsilosis. 8 Apr 1991
• Complete DNA sequence of the linear mitochondrial genome of the pathogenic yeast Candida parapsilosis. 27 Jul 2004
• Several polymers enhance the sensitivity of the southwestern assay. 18 May 1995
• Two acyl-coenzyme A oxidases in peroxisomes of the yeast Candida tropicalis: primary structures deduced from genomic DNA sequence. 27 Feb 1986

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.