Research article summary (published 29 Nov 2007):

Self-inducing shape memory geometric cues embedded within smart hydroxyapatite-based biomimetic matrices.

Full Abstract

BACKGROUND: The authors investigated in nonhuman primates (Papio ursinus) whether it is possible to engineer biomimetic matrices that induce the differentiation of osteoblastic cells expressing selected osteogenic mRNA species of the transforming growth factor (TGF)-beta superfamily. METHODS: Four types of sintered hydroxyapatite and biphasic hydroxyapatite/tricalcium phosphate bioceramics were evaluated as osteoinductive self-inducing biomimetic matrices. Matrices were fabricated with a series of repetitive concavities that initiate the induction of bone formation as a secondary response. Single-phase hydroxyapatite, biphasic hydroxyapatite/tricalcium phosphate, and carbon-impregnated single-phase hydroxyapatite, the latter with fine and coarse porosities, were implanted heterotopically in the rectus abdominis. Specimens for orthotopic calvarial implantation were a total of 16 macroporous disks 25 mm in diameter of single-phase hydroxyapatite and biphasic hydroxyapatite/tricalcium phosphate. RESULTS: Heterotopic specimens 90 and 180 days after implantation showed the induction of bone within concavities of the biomimetic matrices. Northern blot analyses of heterotopic specimens showed that carbon-impregnated single-phase hydroxyapatite specimens induced high expression of osteogenic protein-1 mRNA, correlating with the induction of bone formation. Collagen type IV mRNA was highly expressed, particularly on day 90, by all the implanted matrices. Orthotopic specimens showed substantial bone formation across the implanted constructs. CONCLUSIONS: Self-induced bone has been achieved via the deployment of osteogenic molecular signals expressed by differentiating osteoblastic-like cells, later secreted and embedded into the smart concavities of the biomimetic matrices. The described biomimetic matrices induce de novo bone formation even in the absence of exogenously applied osteogenic proteins of the TGF-beta superfamily.

Author information

Author/s: Ripamonti, Ugo (U); Richter, Paul Wilhelm (PW); Thomas, Michael Edward (ME);

Affiliation: Bone Research Unit, Medical Research Council at the University of the Witwatersrand, Parktown, South Africa. ugo.ripamonti(-atsign-)wits.ac.za

Journal and publication information

Publication Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't

Journal: Plastic and reconstructive surgery (Plast Reconstr Surg), published in United States. (Language: eng)

Reference: 2007-Dec; vol 120 (issue 7) : pp 1796-807

Dates: Created 2007/12/19; Completed 2008/01/22; Revised 2008/11/21;

PMID: 18090741, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article
(including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Bone Morphogenetic Protein 7 Bone Morphogenetic Proteins - biosynthesis; genetics Calcium Phosphates Carbon Ceramics Collagen Type IV - biosynthesis; genetics Durapatite Equipment Design Implants, Experimental Male Materials Testing

Materials Testing Morphogenesis Neovascularization, Physiologic Osseointegration Osteoblasts - cytology; metabolism Papio ursinus Porosity RNA, Messenger - biosynthesis Rectus Abdominis - surgery Skull - surgery Tissue Scaffolds Transforming Growth Factor beta - biosynthesis; genetics

Associated Chemicals: Bone Morphogenetic Protein 7 (0) ; Bone Morphogenetic Proteins (0) ; Calcium Phosphates (0) ; Ceramics (0) ; Collagen Type IV (0) ; RNA, Messenger (0) ; Transforming Growth Factor beta (0) ; Durapatite (1306-06-5) ; Carbon (7440-44-0) ; tricalcium phosphate (7758-87-4)

Related articles

These are the highest related articles currently in the database:

• Enhancement of the in vivo osteogenic potential of marrow/hydroxyapatite composites by bovine bone morphogenetic protein. 13 Dec 2000
• Recombinant human bone morphogenetic protein-2 potentiates the in vivo osteogenic ability of marrow/hydroxyapatite composites. 27 Feb 2001
• Bone tissue induction, using a COLLOSS-filled titanium fibre mesh-scaffolding material. 21 Dec 2004
• Effects of implant surface microtopography on osteoblast gene expression. 29 Nov 2005
• Hierarchically biomimetic bone scaffold materials: nano-HA/collagen/PLA composite. 13 May 2004
• Effects of ion beam-assisted deposition of hydroxyapatite on the osseointegration of endosseous implants in rabbit tibiae. 30 Oct 2001
• Impact of pore size on the vascularization and osseointegration of ceramic bone substitutes in vivo. 30 May 2008
• Compressive forces induce osteogenic gene expression in calvarial osteoblasts. 9 Jan 2008
• Effect of demineralized bone matrix on bone growth within a porous HA material: a histologic and histometric study. 30 Dec 1994
• Mechanism of BMP-2 stimulated adhesion of osteoblastic cells to titanium alloy. 27 Feb 1999

See 100+ related articles.

Related Article Map

Legend: - FREE Full text Article. - Abstract only. - Title only. More help.

See a large map of 100+ related articles.