Simultaneous determination of ginkgolides A, B, C and bilobalide in plasma by LC-MS/MS and its application to the pharmacokinetic study of Ginkgo biloba extract in rats.

Full Abstract

A new liquid chromatography tandem mass spectrometry (LC-MS/MS) method for the simultaneous determination of ginkgolides (includes ginkgolide C for the first time) and bilobalide in plasma is presented. Ketoprofen was used as an internal standard, and sample pre-treatment consisted of a liquid-liquid extraction. Chromatographic separation was achieved on a 5 microm Shiseido C8 column (150 mm x 2.0 mm i.d., particle size 5 microm) with a mobile phase consisting of methanol/ 6 mM ammonium acetate (60/40, v/v) at a flow rate of 0.3 ml/min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring (MRM) under negative ionization mode with an atmospheric pressure chemical ionization (APCI) interface. The method was validated in terms of intra- and inter-day precision (<12.7%), accuracy (within +/- 7.0%), linearity, specificity and stability. In addition, matrix effects of ginkgolides and bilobalide in plasma were evaluated in different reconstitution solvents. Smaller matrix effects were observed for reconstitution solvents containing less organic solvent. The method has been successfully applied to a pharmacokinetic study of Ginkgo biloba extract in rats after intravenous administration. This is the first report of pharmacokinetic data for ginkgolide C.

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Author information

Author/s: Xie, Jiashu (J); Ding, Cungang (C); Ge, Qinghua (Q); Zhou, Zhen (Z); Zhi, Xiaojin (X);

Affiliation: Shanghai Institute of Pharmaceutical Industry, National Pharmaceutical Engineering and Research Center, 1111 Halei Road, Zhangjiang Hi-Tech Park, Shanghai 201203, PR China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences (J Chromatogr B Analyt Technol Biomed Life Sci), published in Netherlands. (Language: eng)

Reference: 2008-Mar; vol 864 (issue 1-2) : pp 87-94

Dates: Created 2008/03/17; Completed 2008/06/05;

PMID: 18295555, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Chromatography, High Pressure Liquid - methods
Cyclopentanes - blood
Female
Furans - blood
Ginkgo biloba - chemistry
Ginkgolides - blood
Lactones - blood

Associated Chemicals: Cyclopentanes (0) ; Furans (0) ; Ginkgolides (0) ; Lactones (0) ; Plant Extracts (0) ; ginkgolide A (0) ; ginkgolide C (15291-76-6) ; bilobalide (33570-04-6) ; ginkgolide B (99796-69-7)

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