A novel workflow control system for Fourier transform ion cyclotron resonance mass spectrometry allows for unique on-the-fly data-dependent decisions.

Full Abstract

In this paper a novel workflow-based data acquisition and control system for Fourier transform ion cyclotron resonance mass spectrometry (FTICR-MS) is presented that facilitates a fast on-the-fly decision-making process for a wide variety of data-dependent experiments. Several new workflow implementations demonstrate the flexibility and benefit of this approach for rapid dynamic experimental design on a chromatographic timescale. The different sequence, evaluation, decision and monitoring modules are described using a selected set of examples. During a tandem liquid chromatography (LC)/FTICR-MS experiment the system is used to dynamically switch between various dissociation techniques such as electron capture dissociation (ECD) and sustained off-resonance irradiation (SORI) depending on the charge state of a tryptic peptide peak. The use of this workflow-based system for imaging FTICR-MS using a desorption electrospray ionization (DESI) source demonstrates the possibility of external control of the workflow by feedback from an imaging sample stage.

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Author information

Author/s: Taban, Ioana M (IM); van der Burgt, Yuri E M (YE); Duursma, Marc (M); Takáts, Zoltán (Z); Seynen, Marco (M); Konijnenburg, Marco (M); Vijftigschild, Anton (A); Attema, Idsart (I); Heeren, Ron M A (RM);

Affiliation: FOM Institute for Atomic and Molecular Physics (FOM-AMOLF), Kruislaan 407, Amsterdam, The Netherlands.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Rapid communications in mass spectrometry : RCM (Rapid Commun Mass Spectrom), published in England. (Language: eng)

Reference: 2008-Apr; vol 22 (issue 8) : pp 1245-56

Dates: Created 2008/04/03; Completed 2008/06/10;

PMID: 18383214, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Peptide Fragments - chemistry
Peptide Mapping
Serine Endopeptidases - chemistry
Spectrometry, Mass, Electrospray Ionization - instrumentation; methods
Spectroscopy, Fourier Transform Infrared - instrumentation; methods
Tandem Mass Spectrometry

Associated Chemicals: Peptide Fragments (0) ; Serine Endopeptidases (EC 3.4.21.-) ; microbial serine proteinases (EC 3.4.21.14)

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