Touchdown PCR, ELISA and stool examination for early diagnosing of Schistosoma mansoni in mice.

Full Abstract

A detection assay for Schistosoma mansoni DNA in mouse serum samples based on touchdown PCR was evaluated. Fifty four mice (7 weeks old) were infected with 60 +/- 10 cercariae of S. mansoni (Egyptian strain) through tail skin. Three days and 1-8 weeks post-infection (PI), six mice were randomly chosen from the infected ones and blood was collected by cardiac puncture. Sera and faeces were collected for 8 weeks PI. Antisoluble egg antigen (SEA) IgG for S. mansoni was detected in infected mice sera of at 3 weeks PI by ELISA. The earliest deposition of eggs in the small intestines was at the 5th week PI. The eggs were detected in faeces at the 8th weeks by modified Kato's thick-smear. However, S. mansoni DNA was detected by touchdown PCR in sera at 2 weeks PI. The intensity of S. mansoni DNA bands in infected mice sera detected in polyacrelamide gel electrophoresis increased gradually during the experimental period. Data recommended touchdown PCR potential tool for the early diagnosis of S. mansoni infection.

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Author information

Author/s: Helmy, Moshira M F (MM);

Affiliation: Department of Parasitology, Medical College, Misr University for Science and Technology, 6th October City, Egypt.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Journal of the Egyptian Society of Parasitology (J Egypt Soc Parasitol), published in Egypt. (Language: eng)

Reference: 2007-Dec; vol 37 (issue 3) : pp 903-13

Dates: Created 2008/04/03; Completed 2008/05/12;

PMID: 18383792, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Antibodies, Helminth - blood
Biomphalaria - parasitology
DNA, Helminth - analysis
Enzyme-Linked Immunosorbent Assay - methods; standards
Feces - microbiology
Humans
Immunoglobulin G - blood

Mice
Parasite Egg Count
Polymerase Chain Reaction - methods; standards
Random Allocation
Schistosoma mansoni - immunology; isolation & purification
Schistosomiasis mansoni - diagnosis
Sensitivity and Specificity
Time Factors

Associated Chemicals: Antibodies, Helminth (0) ; DNA, Helminth (0) ; Immunoglobulin G (0)

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