Use of capillary electrophoresis for polysaccharide studies and applications.

Full Abstract

Capillary electrophoresis (CE) applications to charged polysaccharides are briefly reported. A simple procedure is presented to determine the esterification degree of a hyaluronan derivative. In this case, the degree of substitution was as low as 14%. The molecular weight distribution of mannuronic oligosaccharides mixture produced by hydrolysis of native polymannuronic is readily calculated from peak area of the species resolved by CE on the basis of a specific degree of polymerization. The influence of the applied electric field strength on the free solution mobility of hyaluronan samples is briefly addressed for molar masses of the order of 10(5) and 10(6) g/mol. The data are compared with the results obtained for a 50% galactose-substituted hyaluronic acid (HA). Mobility data obtained as a function of buffer pH for a native HA sample as well as for two galactose-amide HA derivatives, having slightly different degrees of substitution, are presented and discussed in terms of the polymer charge density parameters xi. In most cases, more questions than answers arise from the application of CE to charged polysaccharides. However, perspectives are disclosed for a further understanding of the reliability of CE applied for the structural elucidation of such macromolecules.

Learn Faster Today Improve your study skills

Author information

Author/s: Gamini, Amelia (A); Coslovi, Anna (A); Rustighi, Isabella (I); Campa, Cristiana (C); Vetere, Amedeo (A); Paoletti, Sergio (S);

Affiliation: Department of Biochemistry, Biophysics and Macromolecular Chemistry, University of Trieste, Trieste, Italy.

Journal and publication information

Publication Type: Journal Article

Journal: Methods in molecular biology (Clifton, N.J.) (Methods Mol Biol), published in United States. (Language: eng)

Reference: 2008-; vol 384 (issue ) : pp 357-400

Dates: Created 2008/04/08; Completed 2008/05/07;

PMID: 18392575, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

External Links for this article (including full text providers, if available):

Click Electronic Full-text Provider Links to see options for finding the electronic full text links to this article. Note there may be a subscription or fee required for access to the full text. See our FAQ for information on finding FREE full text articles.

This article may also be located in paper journal collections available in many libraries. Use the Journal and Publication Information above to find the full article.

MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Butyric Acid - analysis
Chromatography, Micellar Electrokinetic Capillary
Electricity
Electrophoresis, Capillary - methods
Esters - analysis

Hyaluronic Acid - analysis
Hydrogen-Ion Concentration
Hydrolysis
Oligosaccharides - analysis
Polysaccharides - analysis
Ultraviolet Rays

Associated Chemicals: Esters (0) ; Oligosaccharides (0) ; Polysaccharides (0) ; Butyric Acid (107-92-6) ; Hyaluronic Acid (9004-61-9)

These are the highest related articles currently in the database:

See 100+ related articles.