Complete genome sequence analysis of dengue virus type 2 isolated in Brunei.

Full Abstract

In a previous study, we have reported the detection and isolation of dengue virus in Brunei (Osman, O., Fong, M.Y., Devi, S., 2007. A preliminary study of dengue infection in Brunei. JJID 60 (4), 205-208). DEN-2 was the predominant serotype followed by DEN-1. The full genomic sequences of 3 DEN-2 viruses isolated during the 2005-2006 dengue incident in Brunei were determined. Twenty-five primer sets were designed to amplify contiguous overlapping fragments of approximately 500-600 base pairs spanning the entire sequence of the viral genome. The amplified PCR products were sent for sequencing and their nucleotides and the deduced amino acids were determined. All three DEN-2 virus isolated were clustered in the Cosmopolitan genotype of the DEN-2 classification by Twiddy et al. This work constitutes the first complete genetic characterization of three Brunei DEN-2 virus strains.

Author information

Author/s: Osman, Osmali (O); Fong, Mun Yik (MY); Devi, Shamala (S);

Affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Malaysia.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Virus research (Virus Res), published in Netherlands. (Language: eng)

Reference: 2008-Jul; vol 135 (issue 1) : pp 48-52

Dates: Created 2008/06/02; Completed 2008/08/28;

PMID: 18406488, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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