Phenolic composition in grape (Vitis vinifera L. cv. Malbec) ripened with different solar UV-B radiation levels by capillary zone electrophoresis.

Full Abstract

The responses of Vitis vinifera L. cv. Malbec to different solar ultraviolet-B radiation (UV-B) levels were assessed in two contrasting situations, under sunlight with full UV-B (+UV-B) and filtered UV-B (-UV-B), in three different locations at 500, 1000, and 1500 m above sea level (asl). To evaluate the effects of radiation, a simple, accurate, and rapid method for the separation and simultaneous determination of representative phenolic compounds in grape berry skins by capillary zone electrophoresis was developed. Separation was carried out in less than 20 min with 20 mM sodium tetraborate buffer containing 30% methanol, pH 9.00. The procedure is fast and reliable, and extracted grape berry skins can be directly analyzed without prior sample cleanup procedure. Berry skins from the +UV-B treatment at 1500 m asl showed the highest levels of total polyphenols anthocyanins, and resveratrol, compared with the -UV-B treatment at this altitude.

Author information

Author/s: Berli, Federico (F); D'Angelo, José (J); Cavagnaro, Bruno (B); Bottini, Rubén (R); Wuilloud, Rodolfo (R); Silva, M Fernanda (MF);

Affiliation: Facultad de Ciencias Agrarias, Universidad Nacional de Cuyo, Alte. Brown 500, 5505 Chacras de Coria, Argentina.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Journal of agricultural and food chemistry (J Agric Food Chem), published in United States. (Language: eng)

Reference: 2008-May; vol 56 (issue 9) : pp 2892-8

Dates: Created 2008/05/07; Completed 2008/07/21;

PMID: 18412357, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

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