Expression of surface CD1d in the extravillous trophoblast cells of early gestational placenta is downregulated in a manner dependent on trophoblast differentiation.

Full Abstract

CD1d is a specific ligand for the invariant Valpha24Vbeta11-natural killer T (iNKT) cells that play an important role in placental development during early human pregnancy. The localization and regulation of placental CD1d expression remain unclear. Immunohistochemistry of human early gestational placentas revealed CD1d was present in villous and extravillous trophoblast (EVT) but not in syncytiotrophoblast or decidual cells. CD1d immunoreactivity in EVT cells decreased with EVT differentiation. Flow cytometry of primary cultured human trophoblast cells confirmed cell-surface expression of CD1d decreased with time in culture. These changes in CD1d expression occur at the level of transcription. TGF-beta1 secreted from the cultured EVT cells accumulated with time in culture and directly suppressed CD1d expression, as evidenced by monoclonal antibody neutralization of TGF-beta1 effects. Thus, trophoblast differentiation is characterized by TGF-beta1-mediated decreases in trophoblast cell CD1d expression. This effect may support appropriate activation of decidual iNKT cells at the maternal-fetal interface.

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Author information

Author/s: Matsumoto, Junko (J); Kawana, Kei (K); Nagamatsu, Takeshi (T); Schust, Danny J (DJ); Fujii, Tomoyuki (T); Sato, Hidetaka (H); Hyodo, Hironobu (H); Yasugi, Toshiharu (T); Kozuma, Shiro (S); Taketani, Yuji (Y);

Affiliation: Department of Obstetrics and Gynecology, Faculty of Medicine, University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8655, Japan.

Journal and publication information

Publication Type: Journal Article

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)

Reference: 2008-Jun; vol 371 (issue 2) : pp 236-41

Dates: Created 2008/05/16; Completed 2008/06/16;

PMID: 18433720, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Antigens, CD1 - analysis; genetics; metabolism
Cell Differentiation
Cells, Cultured
Chorionic Villi - chemistry; immunology
Decidua - immunology
Down-Regulation
Female
Gene Expression Regulation

Gene Expression Regulation
Humans
Killer Cells, Natural - immunology
Lymphocyte Activation
Pregnancy - immunology
Transcription, Genetic
Transforming Growth Factor beta1 - metabolism
Trophoblasts - chemistry; cytology; immunology

Associated Chemicals: Antigens, CD1 (0) ; CD1d antigen (0) ; Transforming Growth Factor beta1 (0)

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