Dietary resveratrol administration increases MnSOD expression and activity in mouse brain.

Full Abstract

trans-Resveratrol (3,4',5-trihydroxystilbene; RES) is of interest for its reported protective effects in a variety of pathologies, including neurodegeneration. Many of these protective properties have been attributed to the ability of RES to reduce oxidative stress. In vitro studies have shown an increase in antioxidant enzyme activities following exposure to RES, including upregulation of mitochondrial superoxide dismutase, an enzyme that is capable of reducing both oxidative stress and cell death. We sought to determine if a similar increase in endogenous antioxidant enzymes is observed with RES treatment in vivo. Three separate modes of RES delivery were utilized; in a standard diet, a high fat diet and through a subcutaneous osmotic minipump. RES given in a high fat diet proved to be effective in elevating antioxidant capacity in brain resulting in an increase in both MnSOD protein level (140%) and activity (75%). The increase in MnSOD was not due to a substantial proliferation of mitochondria, as RES treatment induced a 10% increase in mitochondrial abundance (Citrate Synthase activity). The potential neuroprotective properties of MnSOD have been well established, and we demonstrate that a dietary delivery of RES is able to increase the expression and activity of this enzyme in vivo.

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Author information

Author/s: Robb, Ellen L (EL); Winkelmolen, Lieke (L); Visanji, Naomi (N); Brotchie, Jonathan (J); Stuart, Jeffrey A (JA);

Affiliation: Department of Biological Sciences, Brock University, 500 Glenridge Road, St. Catharines, Ont., Canada.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)

Reference: 2008-Jul; vol 372 (issue 1) : pp 254-9

Dates: Created 2008/06/09; Completed 2008/06/20;

PMID: 18486604, status: MEDLINE (last retrieval date: 11/6/2008)

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals
Antioxidants - administration & dosage
Brain - drug effects; enzymology
Catalase - analysis
Citrate (si)-Synthase - analysis
Diet
Dietary Fats - administration & dosage
Enzyme Activation

Glutathione Peroxidase - analysis
Liver - enzymology
Mice
Mice, Inbred C57BL
Mitochondria - drug effects
Myocardium - enzymology
Stilbenes - administration & dosage
Superoxide Dismutase - analysis; metabolism

Associated Chemicals: Antioxidants (0) ; Dietary Fats (0) ; Stilbenes (0) ; resveratrol (501-36-0) ; Catalase (EC 1.11.1.6) ; Glutathione Peroxidase (EC 1.11.1.9) ; Superoxide Dismutase (EC 1.15.1.1) ; Citrate (si)-Synthase (EC 2.3.3.1)

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