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| Research article summary (published 6 Apr 2008): |
Managing evaporation for more robust microscale assays. Part 1. Volume loss in high throughput assays.
Full Abstract
Evaporation is a well known issue when handling small liquid volumes. Here we present a review of microscale assays prone to evaporation and methods to make them more robust. Applications for these assays span from combinatorial chemistry to cell-biology where the stability of concentrations and osmolarity can be critical. A dimensionless evaporation number Ev is presented and used to characterize volume loss in short term and long term microscale assays. Ev can be used both as a design tool and as an analysis parameter. The advantageous use of evaporation in some applications is also discussed.
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Author information
Author/s: Berthier, Erwin (E); Warrick, Jay (J); Yu, Hongmeiy (H); Beebe, David J (DJ);
Affiliation: MacDiarmid Institute for Advanced Materials and Nanotechnology, Department of Electrical and Computer Engineering, University of Canterbury, Christchurch, NZ. erwin.berthier(-atsign-)gmail.com
Grants: 5T15LM007359 (Agency:United States NLM) ; K25 CA104162 (Agency:United States NCI) ; R21 CA122672 (Agency:United States NCI)
Journal and publication information
Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't; Review
Journal: Lab on a chip (Lab Chip), published in England. (Language: eng)
Reference: 2008-Jun; vol 8 (issue 6) : pp 852-9
Dates: Created 2008/05/23; Completed 2008/08/13;
PMID: 18497901, status: MEDLINE (last retrieval date: 11/6/2008)
Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.
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