Research article summary (published 13 Apr 2008):

Simultaneous determination of 13 bioactive compounds in Herba Artemisiae Scopariae (Yin Chen) from different harvest seasons by HPLC-DAD.

Full Abstract

Herba Artemisiae Scopariae is a Chinese herbal medicine widely used for the remedy of liver diseases. A high performance liquid chromatography method coupled with diode array detection was developed to simultaneously determine 13 different bioactive compounds in Herba Artemisiae Scopariae (Yin Chen) including chlorogenic acid (1), 6,7-dihydroxycoumarin (2), caffeic acid (3), 4-hydroxyacetophenone (4), scopoletin (5), rutin (6), hyperoside (7), isoquercitrin (8), scoparone (11), 7-methoxycoumarine (12) and quercetin (13). By using four different wavelengths in the HPLC analysis, the developed method was able to determine the bioactive compounds with excellent resolution, precision and recovery. The method was applied to determine the amounts of the bioactive compounds in nine samples from different cultivated regions and harvest seasons in China, and significant variations were revealed. Chlorogenic acid was the most abundant among the analyzed compounds. The samples harvested in the spring contained higher contents of chlorogenic acid than those collected in other seasons. Other phenolic acids as caffeic acid, 3,5-dicaffeoylquinic acid, 4,5-dicaffeoylquinic acid and 4-hydroxyacetophenone accumulated at much higher amounts in about May to July. The samples analyzed contained a much lower level of the amount of other flavonoids and coumarins as rutin, hyperoside, isoquercitrin and scoparone.

Author information

Author/s: Tan, Xiao-jie (XJ); Li, Qing (Q); Chen, Xiao-hui (XH); Wang, Zhi-wei (ZW); Shi, Zheng-yuan (ZY); Bi, Kai-shun (KS); Jia, Ying (Y);

Affiliation: School of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Shenyang 110016, Liaoning Province, PR China.

Journal and publication information

Publication Type: Journal Article

Journal: Journal of pharmaceutical and biomedical analysis (J Pharm Biomed Anal), published in England. (Language: eng)

Reference: 2008-Aug; vol 47 (issue 4-5) : pp 847-53

Dates: Created 2008/06/16; Completed 2008/09/03;

PMID: 18502075, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Acetophenones - analysis; chemistry Artemisia - chemistry Caffeic Acids - analysis; chemistry Calibration China Chlorogenic Acid - analogs & derivatives; analysis; chemistry Chromatography, High Pressure Liquid - methods Drug Stability Drugs, Chinese Herbal - analysis Medicine, Chinese Traditional Methanol - chemistry

Methanol - chemistry Molecular Structure Plant Components, Aerial - chemistry Plant Extracts - chemistry; pharmacology Reference Standards Reproducibility of Results Seasons Spectrophotometry, Ultraviolet Temperature Time Factors Water - chemistry

Associated Chemicals: 3,5-dicaffeoylquinic acid (0) ; Acetophenones (0) ; Caffeic Acids (0) ; Drugs, Chinese Herbal (0) ; Plant Extracts (0) ; Chlorogenic Acid (327-97-9) ; caffeic acid (331-39-5) ; Methanol (67-56-1) ; Water (7732-18-5) ; 4-hydroxyacetophenone (99-93-4)

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