Intrinsic bent DNA colocalizes with the sequence involved in the Nd-sD mutation in the Bombyx mori fibroin light chain gene.

Full Abstract

Multiple sequence alignments of the Bombyx mori fibroin light chain gene (fib-L) from hybrids and from Chinese and Japanese strains demonstrated that 51.6% of the fib-L third intron is conserved. One of these conserved segments, 41 bp long, contains the sequence CGTTATTATACATATT, which is duplicated in the B. mori Nd-s(D) mutant. In the present work, electrophoretic mobility assays and computational analyses revealed a major peak of intrinsic bent DNA within the segment that undergoes breakage in the previously-described Nd-s(D) mutation. This result suggested that this intrinsically-curved region might mediate DNA cleavage and enhance recombination events in the third intron of the Bombyx mori fib-L gene.

Author information

Author/s: Barbosa, Joice Felipes (JF); Bravo, Juliana Pereira (JP); Takeda, Karen Izumi (KI); Zanatta, Daniela Bertolini (DB); Silva, Jose Luis da Conceicao (JL); Balani, Valerio Americo (VA); Fiorini, Adriana (A); Fernandez, Maria Aparecida (MA);

Affiliation: Departamento de Biologia Celular e Genetica, Universidade Estadual de Maringa, Maringa, Parana, Brazil. aparecidafernandez(-atsign-)gmail.com

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: BMB reports (BMB Rep), published in Korea (South). (Language: eng)

Reference: 2008-May; vol 41 (issue 5) : pp 394-9

Dates: Created 2008/05/30; Completed 2008/10/14; Revised 2009/04/27;

PMID: 18510871, status: MEDLINE (last retrieval date: 4/27/2009, IMS Date: )

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