Expression of Id and ITF-2 genes in the mammary gland during pregnancy.

Full Abstract

Id-1 is one of the four related helix-loop-helix Id proteins that act as inhibitors of the basic helix-loop-helix (bHLH) transcription factors that control cell differentiation, development and carcinogenesis. We previously found that Id-1 regulated the growth, differentiation, apoptosis and invasion of mouse mammary epithelial cells in culture. Using the techniques of immunohistochemistry and in situ hybridization, we now show that Id-1 gene expression is specifically detected in the epithelial cells of growing ductal structures during early pregnancy. Using adjacent sections, we determined that Id-1 was expressed in keratin 8/18 positive cells. We also demonstrated that the up-regulation of Id-2 during late pregnancy correlated with the down-regulation of Id-1. Using the yeast-two hybrid system, we identified the bHLH transcription factors, ITF-2A and ITF-2B, as the Id-interacting proteins. The levels of expression of these two splice variants did not change during the transition from growing ductal structures to differentiated alveoli. Therefore Id-1 and Id-2, but not the ubiquitous bHLH proteins, appear to represent the key factors whose expression is modulated during different stages of pregnancy in mouse mammary glands.

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Author information

Author/s: Itahana, Yoko (Y); Piens, Marie (M); Fong, Sylvia (S); Singh, Jarnail (J); Sumida, Tomoki (T); Desprez, Pierre-Yves (PY);

Affiliation: California Pacific Medical Center, Cancer Research Institute, 475 Brannan Street, Suite 220, San Francisco, CA 94107, USA.

Grants: R01 CA82548 (Agency:United States NCI)

Journal and publication information

Publication Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't

Journal: Biochemical and biophysical research communications (Biochem Biophys Res Commun), published in United States. (Language: eng)

Reference: 2008-Aug; vol 372 (issue 4) : pp 826-30

Dates: Created 2008/06/25; Completed 2008/07/24;

PMID: 18519032, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Alternative Splicing
Animals
Female
Gene Expression Regulation, Developmental
Helix-Loop-Helix Motifs
Inhibitor of Differentiation Protein 1 - genetics; metabolism
Inhibitor of Differentiation Protein 2 - genetics; metabolism
Mammary Glands, Animal - growth & development; metabolism

Mammary Glands, Animal - growth & development; metabolism
Mice
Mice, Inbred BALB C
Nerve Tissue Proteins - genetics; metabolism
Pregnancy - genetics; metabolism
RNA, Messenger - metabolism
TCF Transcription Factors - genetics; metabolism
Two-Hybrid System Techniques

Associated Chemicals: Idb1 protein, mouse (0) ; Idb2 protein, mouse (0) ; Inhibitor of Differentiation Protein 1 (0) ; Inhibitor of Differentiation Protein 2 (0) ; Nerve Tissue Proteins (0) ; RNA, Messenger (0) ; TCF Transcription Factors (0) ; Tcf4 protein, mouse (0)

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