Research article summary (published 20 Apr 2008):

Characterization of the bovine immunoglobulin lambda light chain constant IGLC genes.

Full Abstract

To characterize the bovine immunoglobulin lambda light chain constant region (IGLC) genes, we have isolated a bacterial artificial chromosome (BAC) clone by a PCR based approach from a bovine genomic DNA library, constructed using a genital ridge cell line derived from a male Holstein fetus. The positive BAC clone, containing the bovine IGLC genes, was fully sequenced and had a 138 kb insert. Sequence analysis revealed that the bovine immunoglobulin lambda light chain locus consisted of four joining-constant gene recombination units spanning approximately 20 kb DNA in length. A detailed examination of the recombination signal sequences, RNA splicing sites and coding sequences of the four joining-constant gene recombination units suggested that only two IGLC genes (IGLC2 and IGLC3) were functional while the IGLC1 and IGLC4 appeared to be pseudogenes. This conclusion was further confirmed by a series of RT-PCR amplifications, which also showed that among these four genes the IGLC3 was preferentially expressed in cattle. Phylogenetic analysis indicated that the bovine IGLC genes were more closely related to their equivalents in sheep and goats than that to other mammals.

Author information

Author/s: Chen, Limei (L); Li, Min (M); Li, Qing (Q); Yang, Xingyuan (X); An, Xiaorong (X); Chen, Yongfu (Y);

Affiliation: State Key Laboratory for Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100094, China.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: Veterinary immunology and immunopathology (Vet Immunol Immunopathol), published in Netherlands. (Language: eng)

Reference: 2008-Aug; vol 124 (issue 3-4) : pp 284-94

Dates: Created 2008/07/21; Completed 2008/10/16;

PMID: 18538861, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Amino Acid Sequence Animals Base Sequence Cattle - genetics; immunology Chromosomes, Artificial, Bacterial Cloning, Molecular DNA - chemistry; genetics Gene Library Genes, Immunoglobulin Light Chain

Immunoglobulin Constant Regions - genetics; immunology Immunoglobulin Joining Region - genetics; immunology Immunoglobulin Light Chains - genetics; immunology Immunoglobulin lambda-Chains - genetics; immunology Male Molecular Sequence Data Phylogeny Polymerase Chain Reaction - veterinary Sequence Alignment

Associated Chemicals: Immunoglobulin Constant Regions (0) ; Immunoglobulin Joining Region (0) ; Immunoglobulin Light Chains (0) ; Immunoglobulin lambda-Chains (0) ; DNA (9007-49-2)

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