Research article summary (published 8 Jun 2008):

Comparative hepatic gene expression profiling of rats treated with 1H,1H,2H,2H-heptadecafluorodecan-1-ol or with pentadecafluorooctanoic acid.

Full Abstract

Pentadecafluorooctanoic acid is an established peroxisome proliferator. Little is known about effects of treatment with 1H,1H,2H,2H-heptadecafluorodecan-1-ol, which is metabolized to pentadecafluorooctanoic acid. We compared effects of various dosages (3, 10, or 25 mg/kg body wt) of each of these compounds on hepatic gene expression in rats with microarrays. Microarray data were validated by real-time RT-PCR. Expression data were also correlated with hepatic activities of selected enzymes and with hepatic levels of pentadecafluorooctanoic acid and 1H,1H,2H,2H-heptadecafluorodecan-1-ol. Pentadecafluorooctanoic acid caused the more powerful change in gene expression, in terms of both number of genes affected and extent of change in expression. Across the dosages used pentadecafluorooctanoic acid and 1H,1H,2H,2H-heptadecafluorodecan-1-ol caused significant (P < or = 0.05) changes in expression for 441 and 105 genes, respectively. With 1H,1H,2H,2H-heptadecafluorodecan-1-ol approximately 38% of the 105 genes exhibited decreased expression with a dose of 25 mg/kg body wt, these genes also appearing less responsive to treatment at the lower dosages. Bioinformatic analysis suggested that these genes are associated with regulatory functions. With pentadecafluorooctanoic acid, increasing dosage up to 10 mg/kg body wt brought about progressive increase in expression of affected genes. Pathways analysis suggested similar effects of the two compounds on lipid and amino acid metabolism. Marked differences were also found, particularly with respect to effects on genes related to oxidative phosphorylation, oxidative metabolism, free radical scavenging, xenobiotic metabolism, and complement and coagulation cascades.

Author information

Author/s: Nilsen, Anja Julie (AJ); Landin, Maria A (MA); Haug, Kristin H (KH); Fonnum, Frode (F); Berger, Urs (U); Osmundsen, Harald (H);

Affiliation: Institute for Oral Biology, University of Oslo, Oslo, Norway.

Journal and publication information

Publication Type: Comparative Study; Journal Article

Journal: Physiological genomics (Physiol Genomics), published in United States. (Language: eng)

Reference: 2008-Aug; vol 34 (issue 3) : pp 285-303

Dates: Created 2008/08/18; Completed 2008/12/16;

PMID: 18544661, status: MEDLINE (last retrieved date: 2/18/2009)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MeSH Headings (categories) shown below.

Animals Cluster Analysis Computational Biology Fluorocarbons - administration & dosage; toxicity Gene Expression Profiling Gene Expression Regulation - drug effects Liver - drug effects; enzymology; metabolism Male Octanoic Acids - administration & dosage; toxicity Oligonucleotide Array Sequence Analysis

Oligonucleotide Array Sequence Analysis Organ Size - drug effects Phenotype RNA, Messenger - genetics; metabolism Rats Rats, Wistar Reproducibility of Results Reverse Transcriptase Polymerase Chain Reaction Signal Transduction - drug effects; genetics Software

Note: Bold headings indicate primary MeSH headings or qualifiers.

Associated Chemicals: Fluorocarbons (0) ; Octanoic Acids (0) ; RNA, Messenger (0) ; perfluorooctanoic acid (335-67-1) ; 1H,1H,2H,2H-perfluorodecanol (678-39-7)

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