Research article summary (published 29 Jun 2008):

Dietary fish oil reverses lipotoxicity, altered glucose metabolism, and nPKCepsilon translocation in the heart of dyslipemic insulin-resistant rats.

Full Abstract

The present study analyzes several markers of energy metabolism in the heart muscle of dyslipemic insulin-resistant rats fed a sucrose-rich diet (SRD, 62.5% wt/wt) for 8 months. It also explores the possible beneficial effects of dietary fish oil supplementation on cardiac lipids and glucose metabolism. With this purpose, male Wistar rats were fed an SRD for 6 months. Whereas half of the animals continued with the same diet for up to 8 months, the other half was fed an SRD in which fish oil (7% + 1% corn oil wt/wt) replaced corn oil (8% wt/wt) from months 6 to 8. The results were compared with rats fed a control diet (starch 62.5% wt/wt). The cardiac muscle of SRD-fed rats showed (1) a significant reduction (P < .05) in key enzymes activities and metabolites involved in glucose metabolism, accompanied by a significant (P < .05) increase of lipid storage (triglyceride, long-chain acyl coenzyme A, and diacylglycerol), and (2) a significant increase (P < .05) of nPKCepsilon protein mass expression in the membrane fraction without changes in the cPKCbetaII. Dietary fish oil, which reduces the availability of plasma lipid flux and normalizes glucose homeostasis, was able to reverse heart muscle lipotoxicity. Fish oil benefits key enzymes activities in glucose metabolism and normalizes glycogen and glucose-6-phosphate concentration, and the altered nPKCepsilon protein mass expression translocation in the heart of SRD-fed rats. Our findings suggest that manipulation of dietary fats may play a key role in the management of lipid disorders, offering a protection against the development of cardiovascular diseases.

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Author information

Author/s: D'Alessandro, María Eugenia (ME); Chicco, Adriana (A); Lombardo, Yolanda B (YB);

Affiliation: Department of Biochemistry, School of Biochemistry, University of Litoral, Ciudad Universitaria, 3000 Santa Fe, Argentina.

Journal and publication information

Publication Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't

Journal: Metabolism: clinical and experimental (Metabolism), published in United States. (Language: eng)

Reference: 2008-Jul; vol 57 (issue 7) : pp 911-9

Dates: Created 2008/06/16; Completed 2008/07/22;

PMID: 18555831, status: MEDLINE (last retrieval date: 11/6/2008)

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Animals Blotting, Western Body Weight - drug effects Corn Oil - pharmacology Diet Dyslipidemias - diet therapy; metabolism Electrophoresis, Polyacrylamide Gel Energy Intake - physiology Fish Oils - pharmacology Glucose - metabolism Glycogen Synthase - metabolism Hexokinase - metabolism

Hexokinase - metabolism Insulin Resistance - physiology Lipids - blood; toxicity Male Myocardium - metabolism Protein Kinase C - metabolism Protein Kinase C-epsilon - metabolism Protein Transport - drug effects Rats Rats, Wistar Subcellular Fractions - drug effects; enzymology Weight Gain - drug effects

Associated Chemicals: Fish Oils (0) ; Lipids (0) ; Glucose (50-99-7) ; Corn Oil (8001-30-7) ; Glycogen Synthase (EC 2.4.1.11) ; protein kinase C beta (EC 2.7.1.-) ; Hexokinase (EC 2.7.1.1) ; Protein Kinase C-epsilon (EC 2.7.1.37) ; Protein Kinase C (EC 2.7.11.13)

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