Research article summary (published 30 Dec 2007):

Sequence analysis of E/NS1 gene junction of dengue virus type 2 isolated in Brunei.

Full Abstract

A preliminary study of dengue infection in Brunei between 2005 and 2006 showed that dengue 2 was the predominant serotype. A total of five DEN-2 isolates were isolated and maintained in the mosquito cell-line, albopictus C6/36. The sequence spanning the envelope and non-structural protein 1 (E/NS1) junction (positions 2311 to 2550) of the isolates were determined and analysed at the amino acid and nucleotide levels. Alignment of the 240 nucleotide sequences among the five isolates showed changes occurring at 7 positions (2.9%) of the region. All but one nucleotide substitution (position 2319, amino acid 742 V --> F) were found at the 3rd position of the codons and were silent mutations. Amino acid homology ranged from 98% to 100%. Sequence divergence of the Brunei isolates varied from 5% to 6.6% compared with dengue-2 prototype New Guinea C strain. Comparison of the Brunei DEN-2 isolates with sixty-five other strains placed them in a cluster containing Indonesian strains isolated in 1973, 1978 and 2004 and Malaysian strains isolated in 1996, 1998 and 1999 in genotype group IV.

Author information

Author/s: Osman, Osmali (O); Fong, Mun Yik (MY); Devi, Shamala (S);

Affiliation: Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.

Journal and publication information

Publication Type: Journal Article; Research Support, Non-U.S. Gov't

Journal: The Southeast Asian journal of tropical medicine and public health (Southeast Asian J Trop Med Public Health), published in Thailand. (Language: eng)

Reference: 2008-Jan; vol 39 (issue 1) : pp 62-78

Dates: Created 2008/06/23; Completed 2008/11/12;

PMID: 18567445, status: MEDLINE (last retrieval date: 2/18/2009, IMS Date: )

Sourced from the National Library of Medicine. Abstract text and other information may be subject to copyright.

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MeSH headings (categories)

This article was linked to the MESH Headings shown below.

Base Sequence Brunei Dengue - genetics; virology Humans

Molecular Sequence Data Sequence Analysis Viral Envelope Proteins - genetics; isolation & purification Viral Nonstructural Proteins - genetics

Associated Chemicals: E-glycoprotein, Dengue virus type 2 (0) ; NS1 protein, Dengue virus type 2 (0) ; Viral Envelope Proteins (0) ; Viral Nonstructural Proteins (0)

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